Table of Contents

Biological Sciences

• Applied Biological Sciences

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    Honeybees use their wings for water surface locomotion

    Chris Roh and Morteza Gharib

    PNAS December 3, 2019 116 (49) 24446-24451; first published November 18, 2019. https://doi.org/10.1073/pnas.1908857116

• Biochemistry

  • Open Access

    A high-affinity human PD-1/PD-L2 complex informs avenues for small-molecule immune checkpoint drug discovery

    Shaogeng Tang and Peter S. Kim

    PNAS December 3, 2019 116 (49) 24500-24506; first published November 14, 2019. https://doi.org/10.1073/pnas.1916916116
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    Structures and single-molecule analysis of bacterial motor nuclease AdnAB illuminate the mechanism of DNA double-strand break resection

    Ning Jia, Mihaela C. Unciuleac, Chaoyou Xue, Eric C. Greene, Dinshaw J. Patel, and Stewart Shuman

    PNAS December 3, 2019 116 (49) 24507-24516; first published November 18, 2019. https://doi.org/10.1073/pnas.1913546116
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    Effects of ALS-associated TANK binding kinase 1 mutations on protein–protein interactions and kinase activity

    Junqiang Ye, Jonah Cheung, Valeria Gerbino, Göran Ahlsén, Christina Zimanyi, David Hirsh, and Tom Maniatis

    PNAS December 3, 2019 116 (49) 24517-24526; first published November 20, 2019. https://doi.org/10.1073/pnas.1915732116
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    Vitamin D binding protein is required to utilize skin-generated vitamin D

    Elizabeth G. Duchow, Nancy E. Cooke, Jeremy Seeman, Lori A. Plum, and Hector F. DeLuca

    PNAS December 3, 2019 116 (49) 24527-24532; first published November 20, 2019. https://doi.org/10.1073/pnas.1915442116
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    Branched unwinding mechanism of the Pif1 family of DNA helicases

    Saurabh P. Singh, Andrea Soranno, Melanie A. Sparks, and Roberto Galletto

    PNAS December 3, 2019 116 (49) 24533-24541; first published November 19, 2019. https://doi.org/10.1073/pnas.1915654116
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    The conserved structure of plant telomerase RNA provides the missing link for an evolutionary pathway from ciliates to humans

    Jiarui Song, Dhenugen Logeswaran, Claudia Castillo-González, Yang Li, Sreyashree Bose, Behailu Birhanu Aklilu, Zeyang Ma, Alexander Polkhovskiy, Julian J.-L. Chen, and Dorothy E. Shippen

    PNAS December 3, 2019 116 (49) 24542-24550; first published November 21, 2019. https://doi.org/10.1073/pnas.1915312116
  • Open Access

    ITPK1 mediates the lipid-independent synthesis of inositol phosphates controlled by metabolism

    Yann Desfougères, Miranda S. C. Wilson, Debabrata Laha, Gregory J. Miller, and Adolfo Saiardi

    PNAS December 3, 2019 116 (49) 24551-24561; first published November 21, 2019. https://doi.org/10.1073/pnas.1911431116

• Biophysics and Computational Biology

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    Clustering and dynamics of crowded proteins near membranes and their influence on membrane bending

    Grzegorz Nawrocki, Wonpil Im, Yuji Sugita, and Michael Feig

    PNAS December 3, 2019 116 (49) 24562-24567; first published November 18, 2019. https://doi.org/10.1073/pnas.1910771116
  • Open Access

    Protein-assisted RNA fragment docking (RnaX) for modeling RNA–protein interactions using ModelX

    Javier Delgado Blanco, Leandro G. Radusky, Damiano Cianferoni, and Luis Serrano

    PNAS December 3, 2019 116 (49) 24568-24573; first published November 15, 2019. https://doi.org/10.1073/pnas.1910999116
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    RNA base-pairing complexity in living cells visualized by correlated chemical probing

    Anthony M. Mustoe, Nicole N. Lama, Patrick S. Irving, Samuel W. Olson, and Kevin M. Weeks

    PNAS December 3, 2019 116 (49) 24574-24582; first published November 19, 2019. https://doi.org/10.1073/pnas.1905491116

• Cell Biology

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    Combined mTORC1/mTORC2 inhibition blocks growth and induces catastrophic macropinocytosis in cancer cells

    Ritesh K. Srivastava, Changzhao Li, Jasim Khan, Nilam Sanjib Banerjee, Louise T. Chow, and Mohammad Athar

    PNAS December 3, 2019 116 (49) 24583-24592; first published November 15, 2019. https://doi.org/10.1073/pnas.1911393116
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    Differentiation of leukemic blasts is not completely blocked in acute myeloid leukemia

    Anupriya Agarwal, William J. Bolosky, David B. Wilson, Christopher A. Eide, Susan B. Olson, Guang Fan, and Brian J. Druker

    PNAS December 3, 2019 116 (49) 24593-24599; first published November 21, 2019. https://doi.org/10.1073/pnas.1904091116
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    Cell adhesion signals regulate the nuclear receptor activity

    Kotaro Sugimoto, Naoki Ichikawa-Tomikawa, Korehito Kashiwagi, Chihiro Endo, Satoshi Tanaka, Norimasa Sawada, Tetsuya Watabe, Tomohito Higashi, and Hideki Chiba

    PNAS December 3, 2019 116 (49) 24600-24609; first published November 18, 2019. https://doi.org/10.1073/pnas.1913346116
  • Open Access

    ROS-mediated PI3K activation drives mitochondrial transfer from stromal cells to hematopoietic stem cells in response to infection

    Jayna J. Mistry, Christopher R. Marlein, Jamie A. Moore, Charlotte Hellmich, Edyta E. Wojtowicz, James G. W. Smith, Iain Macaulay, Yu Sun, Adam Morfakis, Angela Patterson, Rebecca H. Horton, Devina Divekar, Christopher J. Morris, Anna Haestier, Federica Di Palma, Naiara Beraza, Kristian M. Bowles, and Stuart A. Rushworth

    PNAS December 3, 2019 116 (49) 24610-24619; first published November 14, 2019. https://doi.org/10.1073/pnas.1913278116
  • Open Access

    HERES, a lncRNA that regulates canonical and noncanonical Wnt signaling pathways via interaction with EZH2

    Bo-Hyun You, Jung-Ho Yoon, Hoin Kang, Eun Kyung Lee, Sang Kil Lee, and Jin-Wu Nam

    PNAS December 3, 2019 116 (49) 24620-24629; first published November 15, 2019. https://doi.org/10.1073/pnas.1912126116
  • Open Access

    Four-dimensional analyses show that replication compartments are clonal factories in which Epstein–Barr viral DNA amplification is coordinated

    Thejaswi Nagaraju, Arthur U. Sugden, and Bill Sugden

    PNAS December 3, 2019 116 (49) 24630-24638; first published November 19, 2019. https://doi.org/10.1073/pnas.1913992116

• Developmental Biology

  • Open Access

    The proteasome regulator PI31 is required for protein homeostasis, synapse maintenance, and neuronal survival in mice

    Adi Minis, Jose A. Rodriguez, Avi Levin, Kai Liu, Eve-Ellen Govek, Mary E. Hatten, and Hermann Steller

    PNAS December 3, 2019 116 (49) 24639-24650; first published November 21, 2019. https://doi.org/10.1073/pnas.1911921116
  • Open Access

    Glia-derived exosomal miR-274 targets Sprouty in trachea and synaptic boutons to modulate growth and responses to hypoxia

    Yi-Wei Tsai, Hsin-Ho Sung, Jian-Chiuan Li, Chun-Yen Yeh, Pei-Yi Chen, Ying-Ju Cheng, Chun-Hong Chen, Yu-Chen Tsai, and Cheng-Ting Chien

    PNAS December 3, 2019 116 (49) 24651-24661; first published October 30, 2019. https://doi.org/10.1073/pnas.1902537116

    See companion article:

    • Glia modulate growth of the fly neurovascular unit

      - Dec 03, 2019

• Ecology

  • Open Access

    Pervasive decreases in living vegetation carbon turnover time across forest climate zones

    Kailiang Yu, William K. Smith, Anna T. Trugman, Richard Condit, Stephen P. Hubbell, Jordi Sardans, Changhui Peng, Kai Zhu, Josep Peñuelas, Maxime Cailleret, Tom Levanic, Arthur Gessler, Marcus Schaub, Marco Ferretti, and William R. L. Anderegg

    PNAS December 3, 2019 116 (49) 24662-24667; first published November 18, 2019. https://doi.org/10.1073/pnas.1821387116
  • Open Access

    Defense of Scots pine against sawfly eggs (Diprion pini) is primed by exposure to sawfly sex pheromones

    Norbert Bittner, Janik Hundacker, Ander Achotegui-Castells, Olle Anderbrant, and Monika Hilker

    PNAS December 3, 2019 116 (49) 24668-24675; first published November 20, 2019. https://doi.org/10.1073/pnas.1910991116

• Environmental Sciences

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    Production dynamics reveal hidden overharvest of inland recreational fisheries

    Holly S. Embke, Andrew L. Rypel, Stephen R. Carpenter, Greg G. Sass, Derek Ogle, Thomas Cichosz, Joseph Hennessy, Timothy E. Essington, and M. Jake Vander Zanden

    PNAS December 3, 2019 116 (49) 24676-24681; first published November 20, 2019. https://doi.org/10.1073/pnas.1913196116
  • Open Access

    Molybdenum threshold for ecosystem scale alternative vanadium nitrogenase activity in boreal forests

    Romain Darnajoux, Nicolas Magain, Marie Renaudin, François Lutzoni, Jean-Philippe Bellenger, and Xinning Zhang

    PNAS December 3, 2019 116 (49) 24682-24688; first published November 14, 2019. https://doi.org/10.1073/pnas.1913314116
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    Chemical and microbial diversity covary in fresh water to influence ecosystem functioning

    Andrew J. Tanentzap, Amelia Fitch, Chloe Orland, Erik J. S. Emilson, Kurt M. Yakimovich, Helena Osterholz, and Thorsten Dittmar

    PNAS December 3, 2019 116 (49) 24689-24695; first published November 18, 2019. https://doi.org/10.1073/pnas.1904896116

• Evolution

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    Origin of the avian predentary and evidence of a unique form of cranial kinesis in Cretaceous ornithuromorphs

    Alida M. Bailleul, Zhiheng Li, Jingmai O’Connor, and Zhonghe Zhou

    PNAS December 3, 2019 116 (49) 24696-24706; first published November 18, 2019. https://doi.org/10.1073/pnas.1911820116
  • Open Access

    Pollination of Cretaceous flowers

    Tong Bao, Bo Wang, Jianguo Li, and David Dilcher

    PNAS December 3, 2019 116 (49) 24707-24711; first published November 11, 2019. https://doi.org/10.1073/pnas.1916186116
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    Obligate bacterial endosymbionts limit thermal tolerance of insect host species

    Bo Zhang, Sean P. Leonard, Yiyuan Li, and Nancy A. Moran

    PNAS December 3, 2019 116 (49) 24712-24718; first published November 18, 2019. https://doi.org/10.1073/pnas.1915307116
  • Open Access

    BMP signaling inhibition in Drosophila secondary cells remodels the seminal proteome and self and rival ejaculate functions

    Ben R. Hopkins, Irem Sepil, Sarah Bonham, Thomas Miller, Philip D. Charles, Roman Fischer, Benedikt M. Kessler, Clive Wilson, and Stuart Wigby

    PNAS December 3, 2019 116 (49) 24719-24728; first published November 18, 2019. https://doi.org/10.1073/pnas.1914491116
  • Open Access

    The evolution and genomic basis of beetle diversity

    • From the Cover

    Duane D. McKenna, Seunggwan Shin, Dirk Ahrens, Michael Balke, Cristian Beza-Beza, Dave J. Clarke, Alexander Donath, Hermes E. Escalona, Frank Friedrich, Harald Letsch, Shanlin Liu, David Maddison, Christoph Mayer, Bernhard Misof, Peyton J. Murin, Oliver Niehuis, Ralph S. Peters, Lars Podsiadlowski, Hans Pohl, Erin D. Scully, Evgeny V. Yan, Xin Zhou, Adam Ślipiński, and Rolf G. Beutel

    PNAS December 3, 2019 116 (49) 24729-24737; first published November 18, 2019. https://doi.org/10.1073/pnas.1909655116

• Genetics

  • Open Access

    Vertical transmission in Caenorhabditis nematodes of RNA molecules encoding a viral RNA-dependent RNA polymerase

    Aurélien Richaud, Lise Frézal, Stephen Tahan, Hongbing Jiang, Joshua A. Blatter, Guoyan Zhao, Taniya Kaur, David Wang, and Marie-Anne Félix

    PNAS December 3, 2019 116 (49) 24738-24747; first published November 18, 2019. https://doi.org/10.1073/pnas.1903903116

• Immunology and Inflammation

  • Open Access

    Contribution of proteasome-catalyzed peptide cis-splicing to viral targeting by CD8⁺ T cells in HIV-1 infection

    Wayne Paes, German Leonov, Thomas Partridge, Takayuki Chikata, Hayato Murakoshi, Anna Frangou, Simon Brackenridge, Annalisa Nicastri, Andrew G. Smith, Gerald H. Learn, Yingying Li, Robert Parker, Shinichi Oka, Pierre Pellegrino, Ian Williams, Barton F. Haynes, Andrew J. McMichael, George M. Shaw, Beatrice H. Hahn, Masafumi Takiguchi, Nicola Ternette, and Persephone Borrow

    PNAS December 3, 2019 116 (49) 24748-24759; first published November 20, 2019. https://doi.org/10.1073/pnas.1911622116
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    Dichotomous regulation of group 3 innate lymphoid cells by nongastric Helicobacter species

    John W. Bostick, Yetao Wang, Zeli Shen, Yong Ge, Jeffrey Brown, Zong-ming E. Chen, Mansour Mohamadzadeh, James G. Fox, and Liang Zhou

    PNAS December 3, 2019 116 (49) 24760-24769; first published November 18, 2019. https://doi.org/10.1073/pnas.1908128116

• Medical Sciences

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    Proof of concept for identifying cystic fibrosis from perspiration samples

    Zhenpeng Zhou, Daniel Alvarez, Carlos Milla, and Richard N. Zare

    PNAS December 3, 2019 116 (49) 24408-24412; first published November 18, 2019. https://doi.org/10.1073/pnas.1909630116
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    N-acyl taurines are endogenous lipid messengers that improve glucose homeostasis

    Trisha J. Grevengoed, Samuel A. J. Trammell, Michele K. McKinney, Natalia Petersen, Rebecca L. Cardone, Jens S. Svenningsen, Daisuke Ogasawara, Christina C. Nexøe-Larsen, Filip K. Knop, Thue W. Schwartz, Richard G. Kibbey, Benjamin F. Cravatt, and Matthew P. Gillum

    PNAS December 3, 2019 116 (49) 24770-24778; first published November 18, 2019. https://doi.org/10.1073/pnas.1916288116
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    A high-resolution landscape of mutations in the BCL6 super-enhancer in normal human B cells

    Jiang-Cheng Shen, Ashwini S. Kamath-Loeb, Brendan F. Kohrn, Keith R. Loeb, Bradley D. Preston, and Lawrence A. Loeb

    PNAS December 3, 2019 116 (49) 24779-24785; first published November 20, 2019. https://doi.org/10.1073/pnas.1914163116

• Microbiology

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    High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    Carmen Butan, Maria Lara-Tejero, Wenwei Li, Jun Liu, and Jorge E. Galán

    PNAS December 3, 2019 116 (49) 24786-24795; first published November 19, 2019. https://doi.org/10.1073/pnas.1916331116
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    Imaging the dynamic recruitment of monocytes to the blood–brain barrier and specific brain regions during Toxoplasma gondii infection

    Christine A. Schneider, Dario X. Figueroa Velez, Ricardo Azevedo, Evelyn M. Hoover, Cuong J. Tran, Chelsie Lo, Omid Vadpey, Sunil P. Gandhi, and Melissa B. Lodoen

    PNAS December 3, 2019 116 (49) 24796-24807; first published November 14, 2019. https://doi.org/10.1073/pnas.1915778116
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    Self-identity barcodes encoded by six expansive polymorphic toxin families discriminate kin in myxobacteria

    Christopher N. Vassallo and Daniel Wall

    PNAS December 3, 2019 116 (49) 24808-24818; first published November 19, 2019. https://doi.org/10.1073/pnas.1912556116
  • Open Access

    PPARα-targeted mitochondrial bioenergetics mediate repair of intestinal barriers at the host–microbe intersection during SIV infection

    Katti R. Crakes, Clarissa Santos Rocha, Irina Grishina, Lauren A. Hirao, Eleonora Napoli, Christopher A. Gaulke, Anne Fenton, Sandipan Datta, Juan Arredondo, Maria L. Marco, Sumathi Sankaran-Walters, Gino Cortopassi, Cecilia Giulivi, and Satya Dandekar

    PNAS December 3, 2019 116 (49) 24819-24829; first published November 18, 2019. https://doi.org/10.1073/pnas.1908977116

• Neuroscience

  • Open Access

    A locomotor assay reveals deficits in heterozygous Parkinson’s disease model and proprioceptive mutants in adult Drosophila

    Aman Aggarwal, Heinrich Reichert, and K. VijayRaghavan

    PNAS December 3, 2019 116 (49) 24830-24839; first published November 20, 2019. https://doi.org/10.1073/pnas.1807456116
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    Early epigenomic and transcriptional changes reveal Elk-1 transcription factor as a therapeutic target in Huntington’s disease

    Ferah Yildirim, Christopher W. Ng, Vincent Kappes, Tobias Ehrenberger, Siobhan K. Rigby, Victoria Stivanello, Theresa A. Gipson, Anthony R. Soltis, Peter Vanhoutte, Jocelyne Caboche, David E. Housman, and Ernest Fraenkel

    PNAS December 3, 2019 116 (49) 24840-24851; first published November 19, 2019. https://doi.org/10.1073/pnas.1908113116
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    Roles of ErbB3-binding protein 1 (EBP1) in embryonic development and gene-silencing control

    Hyo Rim Ko, Inwoo Hwang, Eun-Ju Jin, Taegwan Yun, Dongryeol Ryu, Jong-Sun Kang, Kye Won Park, Joo-Ho Shin, Sung-Woo Cho, Kyung-Hoon Lee, Keqiang Ye, and Jee-Yin Ahn

    PNAS December 3, 2019 116 (49) 24852-24860; first published November 20, 2019. https://doi.org/10.1073/pnas.1916306116
  • Open Access

    Body map proto-organization in newborn macaques

    Michael J. Arcaro, Peter F. Schade, and Margaret S. Livingstone

    PNAS December 3, 2019 116 (49) 24861-24871; first published November 15, 2019. https://doi.org/10.1073/pnas.1912636116
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    Learning optimal decisions with confidence

    Jan Drugowitsch, André G. Mendonça, Zachary F. Mainen, and Alexandre Pouget

    PNAS December 3, 2019 116 (49) 24872-24880; first published November 15, 2019. https://doi.org/10.1073/pnas.1906787116

• Pharmacology

  • Open Access

    Inhibition of dual-specificity tyrosine phosphorylation-regulated kinase 2 perturbs 26S proteasome-addicted neoplastic progression

    Sourav Banerjee, Tiantian Wei, Jue Wang, Jenna J. Lee, Haydee L. Gutierrez, Owen Chapman, Sandra E. Wiley, Joshua E. Mayfield, Vasudha Tandon, Edwin F. Juarez, Lukas Chavez, Ruqi Liang, Robert L. Sah, Caitlin Costello, Jill P. Mesirov, Laureano de la Vega, Kimberly L. Cooper, Jack E. Dixon, Junyu Xiao, and Xiaoguang Lei

    PNAS December 3, 2019 116 (49) 24881-24891; first published November 21, 2019. https://doi.org/10.1073/pnas.1912033116

• Plant Biology

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    A ligand-independent origin of abscisic acid perception

    Yufei Sun, Ben Harpazi, Akila Wijerathna-Yapa, Ebe Merilo, Jan de Vries, Daphna Michaeli, Maayan Gal, Andrew C. Cuming, Hannes Kollist, and Assaf Mosquna

    PNAS December 3, 2019 116 (49) 24892-24899; first published November 19, 2019. https://doi.org/10.1073/pnas.1914480116
  • Open Access

    The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis

    Takayuki Shimizu, Sylwia M. Kacprzak, Nobuyoshi Mochizuki, Akira Nagatani, Satoru Watanabe, Tomohiro Shimada, Kan Tanaka, Yuuki Hayashi, Munehito Arai, Dario Leister, Haruko Okamoto, Matthew J. Terry, and Tatsuru Masuda

    PNAS December 3, 2019 116 (49) 24900-24906; first published November 15, 2019. https://doi.org/10.1073/pnas.1911251116