Thank you to everybody who answered my query relating to buying a replacement PC for an ABI 3130 sequencer. We have managed to successfully replace our old ABI PC with a similar computer. The following instructions describe how we managed to do this. Thanks Robert robert.donnelly@plymouth.ac.uk 1. A second-hand computer was purchased that met the minimum computer requirements specified by ABI: • Hardware: Pentium® IV Processor, 2.00 GHz Processor • Operating System: Windows XP® Professional Edition • Installed RAM: 1 GB • Hard Disk Storage: Dual 36 GB hard drives • Peripheral: CD/RW 2. A network card was added to the PC (3Com 3C905CX TX M 10 100 PCI Ethernet Network Interface Card) 3. The computer was booted from the original Windows XP CD-Rom. At this stage both hard-drives were partitioned. This created a total of four partitions. 4. After installation of Windows XP, the partitions were formatted in Disk Manager. 5. Network card drivers were then installed. 6. The network card was connected to the Sequencer via an Ethernet cable. 7. A new network connection was created using the IP address 192.168.0.1 and Subnet Mask 255.255.255.0 8. After the sequencer successfully connected to the PC, the data collection software was installed. This required a configuration file to be supplied. 9. With the data collection software installed and running, both the Genemapper and Gene sequencing software was installed Robert Donnelly