Dear All,

A couple weeks ago, I asked the list for suggestions and opinions on
methods for the purification of DNA from agarose gels. Thanks for all the
great responses! Several people asked if I could summarize the results
and send it to the list, so here it is.

I decided to go with the Omega Ultra-Sep Gel Extraction Kit because
it is very cost efficient (500 preps for ~USD $200). Tania King
(who recommended this method, said "it's very cheap and the
yield is very good - better than any other gel kit I've used."
http://www.omegabiotek.com

Other options include:

1. Roche High Pure PCR Purification kit -
used for gel and direct PCR purification
http://www.roche-applied-science.com

2. Bio Rad Freeze 'N Squeeze DNA Gel Extraction
http://www.bio-rad.com

3. Millipore Montage Purification Kit - quick, only takes 10 mins and
no need to melt the gel http://www.millipore.com

4. Healthcare illustra GFX PCR DNA and Gel Band Purification kit
http://www.gelifesciences.com

5. Promega Wizard gel extraction kit
http://www.promega.com

6. Zymoclean Gel DNA recovery Kit from Zymo Research
http://www.zymoresearch.com

7. Qiagen Gel Extraction kits - multiple people reported
having issues with the quality of the extracted products
http://www.qiagen.com

8. "Pepsi's" (published by Travis Glenn, though I couldn't find the ref)
- involves putting aquarium wool in 1mL pipette tips and adding the gel
slice to the wool; spin inside a 1.5mL tube. Recovery varies, but very
cheap (suggested by Joe Stanton)

Stacy J. Mantooth
Ecology and Evolutionary Biology Section
School of Life Sciences
University of Nevada, Las Vegas
4505 Maryland Parkway
Las Vegas, NV 89154-4004
(702) 895-5373 / (702) 895-3956 (fax)

mantooth@unlv.nevada.edu
http://faculty.unlv.edu/mantooth/

mantooth@unlv.nevada.edu