Dear all, I had some interest by other members of evoldir to know the answer to my question regarding the error running MEDUSA in R. Fortunately I had a couple of very useful comments and am able to run it now. Here are the two comments: Hi Ramiro, I was having this same problem, and I found that it has to do with the table layout. In runMedusa, it inserts a column of tip ages (which in your case would be all zeros), but in summaryMedusa it does not insert this same column but the program expects that column in order to run. Its a simple enough problem to fix. I just inserted a column of zeros, but when I mentioned this problem to Luke Harmon, he provided scripts that fixed the issue. I've attached his scripts to this email. Just load these into R and that should fix the problem. Good luck! cheers, Elizabeth Hi Ramiro, This is a bug in the current version of Medusa. Expect a new version soon. In the meantime, you can source the attached files and things should work. Luke Thanks a lot for the replies and hope that it helps other people. Cheers, Ramiro Dr. Ramiro Morales-Hojas Molecular Evolution lab IBMC-Instituto de Biologia Molecular e Celular Rua do Campo Alegre 823 4150-180 Porto Portugal Tel.: +351 226 074 900 extension 1612 rmhojas@ibmc.up.pt rmhojas@ibmc.up.pt