Dear Colleagues, Thank you to everyone who replied. I received 12 replies from researchers and 2 from commercial companies (East Malling and Ecogenics). In summary, 8 commercial companies were recommended by colleagues. GIS, Ecogenics, Newbiotechnique, SREL, Genterprise and Bioprofiles. Both GIS and Ecogenics were the most recommended commercial companies. There were several requests to post the replies so please see below my original request and all the answers I received in order of arrival. I have omitted the two commercial offers I received. Best wishes, Carlos Dear Colleagues, > >We are working on the genetics of invasion of the horse chest nut >leaf-mining moth Cameraria ohridella. >We would like to get a commercial company to develop microsatellites for >us. If anyone knows of a commercial company with a reliable >Microsatellite Development Service please let me know. Thank you. > >Carlos ------ Dear Carlos, we´ve got very good results with www.ecogenics.ch. Cheers and good luck, Nora Dipl. Biol. Nora Brede Dept. Ecology & Evolution Institute for Ecology, Evolution & Diversity ------ Hi Carlos, Sounds like an interesting project. We used a Swiss company called Ecogenics to develop Maculinea microsats, and we were very happy with the result (and the relative price). http://www.ecogenics.ch/ Cheers David Nash David Nash Work: Department of Population Biology, Institute of Biology University of Copenhagen, Universitetsparken 15 DK-2100 Copenhagen Ø, Denmark ------ Hi Carlos, Try Genetic Identification Services in California. I found them to be very efficient. The microsatellites they developed for us were highly polymorphic. They are quite expensive, but worth every cent, with good support afterwards. Check out their website: http://www.genetic-id-services.com/ Good luck, Liz ------ Dear Carlos I suggest Ecogenics (www.ecogenics.ch) - they have a lot of experience and good service. Cheers Alex ETH Zurich Alex Widmer Plant Ecological Genetics Institute of Integrative Biology Universitätstrasse 16 8092 Zurich, Switzerland ------ Dear Carlos. If you want try with this spanish company: Newbiotechique - NBT (Web Page: http://www.newbiotechnic.com/es/) Good luck. Joaquin. Joaquin Munoz Garcia (PhD. Student) Laboratory of Molecular Ecology. Estacion Biologica Donana (CSIC). Pabellon del Peru. Avda. Maria Luisa, s/n. Apartado de Correos 1056. 41013-Sevilla. Spain. ------ These folks provided outstanding service and excellent results. http://www.genetic-id-services.com/ ________________ Michael Sorenson Associate Professor Department of Biology Boston University 5 Cummington St. Boston, MA 02215 ------ Hi Carlos, Our lab is just starting a project with this company: http://www.genetic-id-services.com/library.htm So far, they seem very good. they are easy to contact to get questions answered and they "guarantee" enrichment of an msat library of at least one motif... they have more about this on their website. If not, then the customer does not have to pay anything. good luck! deb ------ Check out the msat development link on the left side of the screen. They're not as fast as other places, but they're good and the cheapest option out there. http://www.uga.edu/srel/DNA_Lab/index.htm Julie ------ Dear Carlos, I was very pleased with the services I received from Genetic Identification Systems (GIS) gisemail@genetic-id-services.com They were very efficient, provided me with both excellent locus identification and primer design, and are have great tech support, including clearly documented protocols and fast response to email queries. I definitely recommend them. --Mark Mark Fishbein Assistant Professor Portland State University Dept. of Biology P.O. Box 751 Portland, OR 97207 ------ Hi Carlos, I made good experiences with Genterprise company located in Mainz/Germany. For my research project on Chironomus midges they developed microsatellite markers fast and really cheap compared to other companys. As the homepage www.genterprise.de is in German, you may better directly contact Prof. Tom Hankeln hankeln@uni-mainz.de . Bye, Carsten Nowak Institute of Ecology, Evolution and Diversity Division of Biosciences J.W.Goethe-University 60054 Frankfurt am Main ------ Hi Carlos, We have very successfully used a company in the US. It’s approx 10,000 US $ for a species (3 motives – incl sequencing of 40 msats containing clones): Genetic Identification Services (www.geneticid-services.com). See our paper on black mangrove (attached) . Cheers Stephan -- Dr Stephan M Funk Conservation Geneticist, Durrell Wildlife Conservation Trust Les Augres Manor, Jersey, JE3 5BP, U.K. Stephan.Funk@durrell.org, http://www.durrellwildlife.org/ Hi carlos Regarding your microsat enquuiry, I highly recommend Bioprofiles at Newcastle, run by Dr Kirsten Wolff. Cheers J Dr. James M. Cook Senior Lecturer Division of Biology Imperial College London Silwood Park Campus Ascot Berks SL5 7PY, UK