PhD position: Genomics and Functional Genetics of Temperature-dependent Egg Development Time in Insects **Application deadline: July 8, 2025** *What?* 4-year full-time PhD position at Leiden University, the Netherlands, with the goal to identify universal genes involved in the temperature sensitivity of insect egg development. To be able to predict which wild insect populations harbor sufficient genetic variation to effectively respond to selection by climate change. *Preferred start date: 1 October 2025* *Why?* Insect species that overwinter as eggs are particularly vulnerable to climate change, as egg development time strongly depends on temperature. However, the genetic underpinning of egg development time in insects is poorly known, making it difficult to assess population adaptive potential. *Who?* You will address this knowledge gap in the group of Maurijn van der Zee at Leiden University, the Netherlands, as part of an NWO ENW M2 grant funded in collaboration with the Department of Animal Ecology at the Netherlands Institute of Ecology (NIOO-KNAW). *How?* You will use functional genetics approaches (RNAi, CRISPR/Cas9) and genomics approaches (RNAseq, WGS) - in both the model system Tribolium castaneum and in wild populations from 12 different insect species (bugs, butterflies, and grasshoppers) - to find universal genes involved in the temperature sensitivity of egg development across insects. *Requirements* MSc in Biology or related field Experience or affinity with analyses of genomic and transcriptomic sequencing data Wet-lab experience with, or eager to learn, molecular biological techniques such as molecular cloning, qPCR, RNAi, and CRISPR/Cas9 Interested to work with insects Strong interest in ecology and evolutionary developmental biology (evo-devo) Ability to work independently, but also to collaborate in an academic research team Skills to communicate scientific data in spoken and written English *Apply now!* For a full description of the position and how to apply, see: https://www.universiteitleiden.nl/vacatures/2025-nl/q2/15758phd-genomics-and-functional-genetics-of-temperature-dependent-egg-development-time-in-insects Or contact Dr. Maurijn van der Zee (m.van.der.zee@biology.leidenuniv.nl) for more information. "Van Dis, Natalie E" (to subscribe/unsubscribe the EvolDir send mail to golding@mcmaster.ca)