Postdoctoral fellow in proteomics and eukaryotic evolution The employment is time-limited for 3 years. The scope is full-time. Location: Uppsala For information about the position, please contact: Fabien Burki Deadline for applying: January 20, 2025 Apply here: https://uu.varbi.com/se/what:job/jobID:778680/ This project is part of an ERC Consolidator grant (PlastidOrigin) awarded to Fabien Burki and aims to study one of the most transformative events in the evolution of eukaryotes: the origin of the photosynthetic organelles (the primary plastids) in the Archaeplastida. The main objective of this postdoctoral position is to create a protein atlas for each organelle of selected red algae and glaucophytes by using whole-cell spatial proteomics with the hyperLOPIT method. By combining these data with verified proteomes of other archaeplastids, and by searching for homologues in other eukaryotes and prokaryotes, we aim to characterize the origin of plastid proteins and the size of the endosymbiotic vs. non-endosymbiotic plastid proteome fractions among the archaeplastids. Duties Maintain knowledge and workflow to perform proteomic experiments in the lab. Use hyperLOPIT on red algae and glaucophytes. Be the contact person for communication with mass spectrometry facilities. Perform routine maintenance of cell cultures. Developing cellular transformation in red algae. Work with high-resolution 3D microscopy data, such as FIB-SEM data. Generate and analyze RNA-seq data.. Grow red algal cultures and run hyperLOPIT. Inferring the cellular location of protein using machine learning. Analyze the data in a plastid development context. Disseminate results in appropriate formats, including in leading peer-reviewed publications and at conferences. Training of junior team members may also be included, as well as other standard tasks related to a research team. Qualification requirements We are looking for you who either have a degree (minimum PhD) in a relevant field such as biology, chemistry or equivalent. The degree must be completed at the latest at the time when the employment decision is made. Primarily, those who have completed their degree no more than three years ago should be considered. When calculating the time frame of three years, the starting point is the application deadline. If there are special reasons, such a degree may have been completed earlier. Special reasons refer to leave due to illness, parental leave, positions of trust within trade unions, etc. We are looking for an enthusiastic person with the desire to share and establish a new methodology in the Burki lab. The successful candidate should have a solid list, appropriate for their career stage, of high-quality research publications. Great importance is placed on personal qualities such as high motivation, planning and organizational skills, problem solving, stress management. We strive to create a friendly atmosphere among team members and other employees in the workplace, so a collegial ability to work as part of a team is necessary. For this position, proven experience in mass spectrometry and proteomics is imperative, and must be demonstrated through scientific publications in these areas. The applicant should also have documented skills in wet lab techniques, such as molecular biology (ie, PCR, cloning, Western blot, SDS-PAGE, protein/DNA/RNA extractions), cellular transformation, and cell culture. Previous experience with 3D image analysis, such as FIB-SEM data, will be considered a valuable asset. Excellent statistical analysis and bioinformatics skills (especially in R) are required, and previous experience with R packages for spatial proteomics analysis, such as pRoloc, is highly beneficial. Prior knowledge of methods of subcellular fractionation (using ultracentrifugation) and of hyperLOPIT or LOPIT-DC will be seen as a great asset. A strong interest in evolution and microbial eukaryotes is a desirable characteristic of the candidates. Knowledge of English, both oral and written, is a requirement. The candidate should be willing to travel, for shorter (eg conference) or longer periods if training is necessary. N�r du har kontakt med oss p� Uppsala universitet med e-post s� inneb�r det att vi behandlar dina personuppgifter. F�r att l�sa mer om hur vi g�r det kan du l�sa h�r: http://www.uu.se/om-uu/dataskydd-personuppgifter/ E-mailing Uppsala University means that we will process your personal data. For more information on how this is performed, please read here: http://www.uu.se/en/about-uu/data-protection-policy Fabien Burki (to subscribe/unsubscribe the EvolDir send mail to golding@mcmaster.ca)