Dear All,

EMBO Practical Course on Genome sequencing, assembly, curation, and
downstream analyses

Location: Florence, Italy
Dates: 8-12 September, 2025
Application deadline: 31 May, 2025
EMBO travel grants and registration fee waivers are available

This hands-on course, supported by the European Molecular Biology
Organization, will guide participants through main sequencing techniques
and latest bioinformatic tools to produce high-quality genome assemblies
of interest for downstream analysis and publication.

The course offers practical training in the use of computational tools
for genome assembly such as HiFiasm, Verkko, Flye and NextDeNovo for
PacBio HiFi reads or Flye, Canu, Raven and NextDeNovo for ONT reads,
with troubleshooting sessions highlighting strengths and weaknesses of
each methodology. Training and learning tools include a user-friendly
dedicated Galaxy instance (https://usegalaxy.eu) through the Galaxy
Training Infrastructure as a Service (TIaaS, https://usegalaxy.org/tiaas),
which provides a GUI to run and analyse complex workflow. Students
will be also trained in the use and interpretation of tools to perform
Quality Control at each step of the genome assembly process and will
participate in discussion on the quality of the results and possible
strategies to improve the accuracy of assemblies. The course is aimed
at graduate students and postdoctoral researchers as well as scientists
at any stage of their careers.

Speakers and Instructors:

Astrid B�hne, Museum Koenig Bonn, DE
Aureliano Bombarely, CSIC Institute for Plant Molecular and Cellular
Biology, ES
Tom Brown, Leibniz Institute for Zoo and Wildlife Research, DE
Claudio Ciofi, University of Florence, IT
Jean-Fran�ois Flot, Universit� libre de Bruxelles, BE
Giulio Formenti, The Rockefeller University, USA
Bj�rn Gr�ning, University of Freiburg, DE
Nad�ge Guiglielmoni, University of Cologne, DE
Silvia Manrique, Polytechnic University of Valencia, ES
Kirsty McCaffrey, The Rockefeller University, USA
Alice Mouton, University of Li�ge, BE
Marco Sollitto, University of Florence, IT

***General information can be found on the EMBO course website:
https://meetings.embo.org/event/25-genome-seq

***Online applications can be submitted here:
https://coms.app/pc25-33/welcome.html

On behalf of the coordination team
Claudio Ciofi

Claudio Ciofi
Professor of Ecology and Molecular Ecology
Associate Head
Department of Biology
University of Florence
Via Madonna del Piano 6, Sesto Fiorentino (FI) 50019, ITALY
Tel: +39 055 457 4740

Claudio CIofi <claudio.ciofi@unifi.it>

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