Dear all,

registrations are open for the 2nd edition of the online course
"Introduction to CRISPR for Ecology and Evolution Studies".

Dates: 14-18 October

Course website: (
https://www.physalia-courses.org/courses-workshops/course53/ )

The course is intended for researchers interested in implementing
the CRISPR-Cas9 tool in their own projects. There will be theoretical
lectures and hands-on practical exercises using web-based tools,
license-free software, and UNIX-based command line programs.
Experience using command-line based programs will be advantageous,
but not required. An introduction to UNIX commands will be provided.

At the end of this course, attendees will be in the position to:

- Understand the fundamentals of the CRISPR-Cas9 genome-editing tool.
- Design a reliable CRISPR-based gene knockout experiment.
- Functionally investigate a gene of interest in a non-model species.
- Design and evaluate species-specific guide RNAs (gRNAs).
- Efficiently synthesize gRNA and assemble optimized Cas9/gRNA mixes.
- Screen for CRISPR-derived mutants using a number of genotyping methods.
- Design genotyping strategies according to project needs.
- Analyze Cas9-induced genomic variants by Illumina deep sequencing.
- Critically interpret the results from a CRISPR-based KO experiment

For the full list of our courses and workshops, please visit: (
https://www.physalia-courses.org/courses-workshops/course53/ )

Should you have any questions, please do not hesitate to contact
us.

Best regards, Carlo

Carlo Pecoraro, Ph.D
Physalia-courses DIRECTOR
info@physalia-courses.org
mobile: +49 17645230846

"info@physalia-courses.org" <info@physalia-courses.org>

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