Dear all, We are excited to announce our upcoming online course, Assembly and Annotation of Genomes, scheduled for 17-21 March 2025. Course website: ( https://www.physalia-courses.org/courses-workshops/course20/ ) This course provides a comprehensive introduction to de novo genome assembly and annotation, covering the latest sequencing technologies (e.g., Illumina, PacBio, Oxford Nanopore) and scaffolding methods (e.g., Hi-C, optical mapping). Participants will learn hands-on techniques for assembly evaluation, visualization, and feature annotation. Key highlights: - Understand the concepts related to de novo genome assembly and annotation for genomes of all sizes, from viruses to mammals - Learn the strengths and weaknesses of different sequencing technologies, including Illumina short read sequencing, Pacific Biosciences and Oxford Nanopore long read sequencing, as well as scaffolding technologies including optical mapping and proximity ligation (Hi-C), for de novo genome assembly and annotation. - Gain hands on experience with common tools for de novo genome assembly, assembly quality evaluation, assembly visualization and manual curation - Hands on experience of feature annotation (e.g. genes, repeats) Designed for researchers at all levels no prior sequencing background required. For the full list of our courses and workshops, please visit: ( https://www.physalia-courses.org/courses-workshops/course20/ ) Best regards, Carlo Carlo Pecoraro, Ph.D Physalia-courses DIRECTOR info@physalia-courses.org mobile: +49 17645230846 "info@physalia-courses.org" (to subscribe/unsubscribe the EvolDir send mail to golding@mcmaster.ca)