Dear all, registrations are open for the 5th edition of the online course on the Analysis of RNAseq data with R/Bioconductor: ( https://www.physalia-courses.org/courses-workshops/course19/ ) This course will provide biologists and bioinformaticians with practical statistical analysis skills to perform rigorous analysis of high-throughput genomic data. The course assumes basic familiarity with genomics and with R programming, but does not assume prior statistical training. It covers the statistical concepts necessary to analyze genomic and transcriptomic high-throughput data generated by next-generation sequencing, including: hypothesis testing, data visualization, genomic region analysis, differential expression analysis, and gene set analysis. The course takes place over 6 days, with each session lasting 3 hours: ** Session 1 - Introduction (Nov 4, 12-3 PM, Berlin time) - Introduction to R / RStudio - Creating high-quality graphics in R ** Session 2 - Hypothesis testing (Nov 6, 12-3 PM, Berlin time) - CDF, p-value, binomial test - types of error, t-test, permutation test ** Session 3 - Introduction to Bioconductor (Nov 8, 12-3 PM, Berlin time) - Introduction to Bioconductor - Working with genomic region data in Bioconductor (GenomicRanges) ** Session 4 - RNA-seq data analysis (Nov 11, 12-3 PM, Berlin time) - Characteristics of RNA-seq data - Storing and analyzing RNA-seq data in Bioconductor (SummarizedExperiment) ** Session 5 - Differential expression analysis (Mon, Nov 13, 12-3 PM, Berlin time) - Multiple hypothesis testing - Performing differential expression analysis with DESeq2 ** Session 6 - Gene set analysis (Wed, Nov 15, 12-3 PM, Berlin time) - A primer on terminology, existing methods & statistical theory - GO/KEGG overrepresentation analysis - Functional class scoring & permutation testing For the full list of our courses and workshops, please visit: ( https://www.physalia-courses.org/courses-workshops/course19/ ) Best regards, Carlo Carlo Pecoraro, Ph.D Physalia-courses DIRECTOR info@physalia-courses.org mobile: +49 17645230846 "info@physalia-courses.org" (to subscribe/unsubscribe the EvolDir send mail to golding@mcmaster.ca)