Current Issue | PLOS Biology

Research on bacteriophages, the viruses infecting bacteria, has fueled the development of modern molecular biology and inspired their therapeutic application to combat bacterial multidrug resistance. However, most work has so far focused on a few model phages which impedes direct applications of these findings in clinics and suggests that a vast potential of powerful molecular biology has remained untapped. Humolli, Piel et al. have therefore recently compiled the BASEL collection of Escherichia coli phages (BActeriophage SElection for your Laboratory), which made a relevant diversity of phages infecting the E. coli K-12 laboratory strain accessible to the community. These phages are widely used, but their assorted diversity has remained limited by the E. coli K-12 host. The authors have therefore now genetically overcome the two major limitations of E. coli K-12, namely its lack of O-antigen glycans and the presence of resident bacterial immunity. Restoring O-antigen expression resulted in the isolation of diverse additional viral groups like Kagunavirus, Nonanavirus, Gordonclarkvirinae, and Gamaleyavirus, while eliminating all known antiviral defenses of E. coli K-12 additionally enabled the isolation of phages of the Wifcevirus genus. Even though some of these viral groups appear to be common in nature, no phages from any of them had previously been isolated using E. coli laboratory strains, and they had thus remained largely understudied. Overall, 37 new phage isolates have been added to complete the BASEL collection. The image shows four of these phages superimposed on a map of central Basel.

Image Credit: Fabienne Estermann

Editorial

Vaccines work… and do not cause autism

Nonia Pariente

PLOS Biology: published April 18, 2025 | https://doi.org/10.1371/journal.pbio.3003143

Unsolved Mystery

How did vocal communication come to dominate human language? A view from the womb

Alexis Hervais-Adelman, Simon W. Townsend

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003141

Perspectives

The end of long-term ecological data?

Daniel T. Blumstein

PLOS Biology: published April 3, 2025 | https://doi.org/10.1371/journal.pbio.3003102

Priorities for rapid and cost-effective vaccines to improve outbreak responses

Sarah C. Gilbert

PLOS Biology: published April 7, 2025 | https://doi.org/10.1371/journal.pbio.3003114

One-for-one or one-for-all? Considerations for filovirus vaccine development

Andrea Marzi

PLOS Biology: published April 14, 2025 | https://doi.org/10.1371/journal.pbio.3003142

Applications and status of gene drive in plants

Bruce A. Hay

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003148

Primers

Glial cells diverge in fly brain evolution

Yaoyu Jiao, Trevor R. Sorrells

PLOS Biology: published April 30, 2025 | https://doi.org/10.1371/journal.pbio.3003136

Ploidy plasticity drives fungal resistance to azoles used in agriculture and clinics

Kaustuv Sanyal, Aswathy Narayanan

PLOS Biology: published April 2, 2025 | https://doi.org/10.1371/journal.pbio.3003083

A single enzyme becomes a Swiss Army knife

Andreas Sichert

PLOS Biology: published April 2, 2025 | https://doi.org/10.1371/journal.pbio.3003072

Refining the sleep circuits one neuron at a time

Giorgio F. Gilestro

PLOS Biology: published April 4, 2025 | https://doi.org/10.1371/journal.pbio.3003101

The innate immune system: A double-edged sword

Ditte S. Andersen, Julien Colombani

PLOS Biology: published April 29, 2025 | https://doi.org/10.1371/journal.pbio.3003088

Vps34 puts the ‘e’ in eTreg cells

Ruchi Saxena, You-Wen He

PLOS Biology: published April 14, 2025 | https://doi.org/10.1371/journal.pbio.3003109

A hypermobile prophage in the genome of a key human gut bacterium

Andrey N. Shkoporov, Colin Hill

PLOS Biology: published April 1, 2025 | https://doi.org/10.1371/journal.pbio.3003071

Research Articles

Comparative single-cell transcriptomic atlases of drosophilid brains suggest glial evolution during ecological adaptation

Daehan Lee, Michael P. Shahandeh, Liliane Abuin, Richard Benton

PLOS Biology: published April 29, 2025 | https://doi.org/10.1371/journal.pbio.3003120

Diatom heterotrophy on brown algal polysaccharides emerged through horizontal gene transfer, gene duplication, and neofunctionalization

Zeng Hao Lim, Peng Zheng, Christopher Quek, Minou Nowrousian, Finn L. Aachmann, Gregory Jedd

PLOS Biology: published April 1, 2025 | https://doi.org/10.1371/journal.pbio.3003038

An agricultural triazole induces genomic instability and haploid cell formation in the human fungal pathogen Candida tropicalis

Tianren Hu, Qiushi Zheng, Chengjun Cao, Shuaihu Li, Yanfeng Huang, Zhangyue Guan, Lingyu Ji, Jian Bing, Han Du, Austin M. Perry, Clarissa J. Nobile, Bing Li, Haiqing Chu, Guanghua Huang

PLOS Biology: published April 1, 2025 | https://doi.org/10.1371/journal.pbio.3003062

The multiscale brain structural re-organization that occurs from childhood to adolescence correlates with cortical morphology maturation and functional specialization

Yirong He, Debin Zeng, Qiongling Li, Lei Chu, Xiaoxi Dong, Xinyuan Liang, Lianglong Sun, Xuhong Liao, Tengda Zhao, Xiaodan Chen, Tianyuan Lei, Weiwei Men, Yanpei Wang, Daoyang Wang, Mingming Hu, Zhiying Pan, Haibo Zhang, Ningyu Liu, Shuping Tan, Jia-Hong Gao, Shaozheng Qin, Sha Tao, Qi Dong, Yong He, Shuyu Li

PLOS Biology: published April 1, 2025 | https://doi.org/10.1371/journal.pbio.3002710

Reactivation of previous decisions repulsively biases sensory encoding but attractively biases decision-making

Minghao Luo, Huihui Zhang, Fang Fang, Huan Luo

PLOS Biology: published April 23, 2025 | https://doi.org/10.1371/journal.pbio.3003150

Feedback from an avatar facilitates risk-taking by modulating the amygdala response to feedback uncertainty

Toshiko Tanaka, Masahiko Haruno

PLOS Biology: published April 22, 2025 | https://doi.org/10.1371/journal.pbio.3003122

Top-down and bottom-up interactions rely on nested brain oscillations to shape rhythmic visual attention sampling

Jelena Trajkovic, Domenica Veniero, Simon Hanslmayr, Satu Palva, Gabriela Cruz, Vincenzo Romei, Gregor Thut

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3002688

Dorsomedial and ventromedial prefrontal cortex lesions differentially impact social influence and temporal discounting

Zhilin Su, Mona M. Garvert, Lei Zhang, Todd A. Vogel, Jo Cutler, Masud Husain, Sanjay G. Manohar, Patricia L. Lockwood

PLOS Biology: published April 28, 2025 | https://doi.org/10.1371/journal.pbio.3003079

The dorsal and ventral hippocampus contribute differentially to spatial working memory and spatial coding in the prefrontal cortex

Susanne S. Babl, Torfi Sigurdsson

PLOS Biology: published April 23, 2025 | https://doi.org/10.1371/journal.pbio.3003140

The zona incerta negatively regulates the red nucleus during movement cued by sound signals

Liang Chen, Xinxing Wang, Hanxiao Liu, Chenzhao He, Allen P. F. Chen, Lu Chen, Thomas A. Kim, Qiaojie Xiong

PLOS Biology: published April 7, 2025 | https://doi.org/10.1371/journal.pbio.3003092

Whisking and locomotion are jointly represented in superior colliculus neurons

Suma Chinta, Scott R. Pluta

PLOS Biology: published April 7, 2025 | https://doi.org/10.1371/journal.pbio.3003087

Active and passive touch are differentially represented in the mouse somatosensory thalamus

Anton Sumser, Emilio Ulises Isaías-Camacho, Rebecca Audrey Mease, Alexander Groh

PLOS Biology: published April 8, 2025 | https://doi.org/10.1371/journal.pbio.3003108

Purkinje cells of the cerebellum control deceleration of tongue movements

Paul Hage, Mohammad Amin Fakharian, Alden M. Shoup, Jay S. Pi, Ehsan Sedaghat-Nejad, Simon P. Orozco, In Kyu Jang, Vivian Looi, Hisham Y. Elseweifi, Nazanin Mohammadrezaei, Alexander N. Vasserman, Toren Arginteanu, Reza Shadmehr

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3003110

Inhibitory circuit motifs in Drosophila larvae generate motor program diversity and variability

Jacob Francis, Caius R. Gibeily, William V. Smith, Isabel S. Petropoulos, Michael Anderson, William J. Heitler, Astrid A. Prinz, Stefan R. Pulver

PLOS Biology: published April 21, 2025 | https://doi.org/10.1371/journal.pbio.3003094

Modulation of NMDA receptor signaling and zinc chelation prevent seizure-like events in a zebrafish model of SLC13A5 epilepsy

Deepika Dogra, Van Anh Phan, Sinan Zhang, Cezar Gavrilovici, Nadia DiMarzo, Ankita Narang, Kingsley Ibhazehiebo, Deborah M. Kurrasch

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3002499

Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice

Camille Quilgars, Eric Boué-Grabot, Philippe de Deurwaerdère, Jean-René Cazalets, Florence E. Perrin, Sandrine S. Bertrand

PLOS Biology: published April 21, 2025 | https://doi.org/10.1371/journal.pbio.3003153

The G protein modifier KCTD5 tunes the decoding of neuromodulatory signals necessary for motor function in striatal neurons

Douglas C. Sloan, Yini Liao, Forest Ray, Brian S. Muntean

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003117

Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice

Dongdong Zhao, Yuanhui Huo, Naizhen Zheng, Xiang Zhu, Dingting Yang, Yunqiang Zhou, Shengya Wang, Yiru Jiang, Yili Wu, Yun-wu Zhang

PLOS Biology: published April 1, 2025 | https://doi.org/10.1371/journal.pbio.3003047

Total cerebral blood volume changes drive macroscopic cerebrospinal fluid flux in humans

Juliana Zimmermann, Clara Boudriot, Christiane Eipert, Gabriel Hoffmann, Rachel Nuttall, Viktor Neumaier, Moritz Bonhoeffer, Sebastian Schneider, Lena Schmitzer, Jan Kufer, Stephan Kaczmarz, Dennis M Hedderich, Andreas Ranft, Daniel Golkowski, Josef Priller, Claus Zimmer, Rüdiger Ilg, Gerhard Schneider, Christine Preibisch, Christian Sorg, Benedikt Zott

PLOS Biology: published April 24, 2025 | https://doi.org/10.1371/journal.pbio.3003138

Werner syndrome exonuclease promotes gut regeneration and causes age-associated gut hyperplasia in Drosophila

Kun Wu, Juanyu Zhou, Yiming Tang, Qiaoqiao Zhang, Lishou Xiong, Xiaorong Li, Zhangpeng Zhuo, Mei Luo, Yu Yuan, Xingzhu Liu, Zhendong Zhong, XiaoXin Guo, Zihua Yu, Xiao Sheng, Guanzheng Luo, Haiyang Chen

PLOS Biology: published April 22, 2025 | https://doi.org/10.1371/journal.pbio.3003121

NF-κB signaling driven by oncogenic Ras contributes to tumorigenesis in a Drosophila carcinoma model

Caroline Dillard, José Teles-Reis, Ashish Jain, Marina Gonçalves Antunes, Paula Ruiz-Duran, Yanyan Qi, Roland Le Borgne, Heinrich Jasper, Tor Erik Rusten

PLOS Biology: published April 28, 2025 | https://doi.org/10.1371/journal.pbio.3002663

Hedgehog-stimulated phosphorylation at multiple sites activates Ci by altering Ci–Ci interfaces without full Suppressor of Fused dissociation

Hoyon Kim, Jamie C. Little, Jiashen Li, Bryna Patel, Daniel Kalderon

PLOS Biology: published April 11, 2025 | https://doi.org/10.1371/journal.pbio.3003105

Gas1-high quiescent neural stem cells are multipotent and produce oligodendrocytes during aging and after demyelinating injury

Chaoqiong Ding, Zhenzhong Pan, Xiang Yan, Ran Zhou, Huifang Li, Lu Chen, Yuan Wang, Yan Zhang

PLOS Biology: published April 3, 2025 | https://doi.org/10.1371/journal.pbio.3003100

Polarized subcellular activation of Rho proteins by specific ROPGEFs drives pollen germination in Arabidopsis thaliana

Alida Melissa Bouatta, Franziska Anzenberger, Lisa Riederauer, Andrea Lepper, Philipp Denninger

PLOS Biology: published April 21, 2025 | https://doi.org/10.1371/journal.pbio.3003139

Single-cell analysis of the early Drosophila salivary gland reveals that morphogenetic control involves both the induction and exclusion of gene expression programs

Annabel May, Katja Röper

PLOS Biology: published April 21, 2025 | https://doi.org/10.1371/journal.pbio.3003133

The length of the G1 phase is an essential determinant of H3K27me3 landscapes across diverse cell types

Abby Trouth, Kameswaran Ravichandran, Philip R. Gafken, Sara Martire, Gabriel E. Boyle, Giovana M. B. Veronezi, Van La, Stephanie J. Namciu, Laura A. Banaszynski, Jay F. Sarthy, Srinivas Ramachandran

PLOS Biology: published April 17, 2025 | https://doi.org/10.1371/journal.pbio.3003119

Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation

Md. Hashim Reza, Rashi Aggarwal, Jigyasa Verma, Nitesh Kumar Podh, Ratul Chowdhury, Gunjan Mehta, Ravi Manjithaya, Kaustuv Sanyal

PLOS Biology: published April 2, 2025 | https://doi.org/10.1371/journal.pbio.3003069

TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the Golgi

Elsa Ronzier, Prasanna Satpute-Krishnan

PLOS Biology: published April 9, 2025 | https://doi.org/10.1371/journal.pbio.3003084

Phase separation of the PRPP amidotransferase into dynamic condensates promotes de novo purine synthesis in yeast

Masak Takaine, Rikuri Morita, Yuto Yoshinari, Takashi Nishimura

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3003111

Variant mutation G215C in SARS-CoV-2 nucleocapsid enhances viral infection via altered genomic encapsidation

Hannah C. Kubinski, Hannah W. Despres, Bryan A. Johnson, Madaline M. Schmidt, Sara A. Jaffrani, Allyson H. Turner, Conor D. Fanuele, Margaret G. Mills, Kumari G. Lokugamage, Caroline M. Dumas, David J. Shirley, Leah K. Estes, Andrew Pekosz, Jessica W. Crothers, Pavitra Roychoudhury, Alexander L. Greninger, Keith R. Jerome, Bruno Martorelli Di Genova, David H. Walker, Bryan A. Ballif, Mark S. Ladinsky, Pamela J. Bjorkman, Vineet D. Menachery, Emily A. Bruce

PLOS Biology: published April 29, 2025 | https://doi.org/10.1371/journal.pbio.3003115

A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth

Iratxe Zuazo-Gaztelu, David Lawrence, Ioanna Oikonomidi, Scot Marsters, Ximo Pechuan-Jorge, Catarina J. Gaspar, David Kan, Ehud Segal, Kevin Clark, Maureen Beresini, Marie-Gabrielle Braun, Joachim Rudolph, Zora Modrusan, Meena Choi, Wendy Sandoval, Mike Reichelt, David C. DeWitt, Pekka Kujala, Suzanne van Dijk, Judith Klumperman, Avi Ashkenazi

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3003086

Staphylococcal toxin PVL ruptures model membranes under acidic conditions through interactions with cardiolipin and phosphatidic acid

Seong H. Chow, Yusun Jeon, Pankaj Deo, Amy T. Y. Yeung, Christine Hale, Sushmita Sridhar, Gilu Abraham, Joshua Nickson, Françios A. B. Olivier, Jhih-Hang Jiang, Yue Ding, Mei-Ling Han, Anton P. Le Brun, Dovile Anderson, Darren Creek, Janette Tong, Kip Gabriel, Jian Li, Ana Traven, Gordon Dougan, Hsin-Hui Shen, Thomas Naderer

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003080

Interferon regulatory factor 4 mediates nonenzymatic IRE1 dependency in multiple myeloma cells

Ioanna Oikonomidi, Vasumathi Kameswaran, Victoria C. Pham, Iratxe Zuazo-Gaztelu, Lauren M. Gutgesell, Scot Marsters, Bence Daniel, Jennie R. Lill, Zora Modrusan, Avi Ashkenazi

PLOS Biology: published April 11, 2025 | https://doi.org/10.1371/journal.pbio.3003096

Vps34-orchestrated lipid signaling processes regulate the transitional heterogeneity and functional adaptation of effector regulatory T cells

Erienne G. Norton, Nicole M. Chapman, Hao Shi, Xiaoxi Meng, Hongling Huang, Anil KC, Sherri Rankin, Jordy Saravia, Sujing Yuan, Haoran Hu, Peter Vogel, Hongbo Chi

PLOS Biology: published April 11, 2025 | https://doi.org/10.1371/journal.pbio.3003074

Liver gene expression and its rewiring in hepatic steatosis are controlled by PI3Kα-dependent hepatocyte signaling

Marion Régnier, Arnaud Polizzi, Tiffany Fougeray, Anne Fougerat, Prunelle Perrier, Karen Anderson, Yannick Lippi, Sarra Smati, Céline Lukowicz, Frédéric Lasserre, Edwin Fouche, Marine Huillet, Clémence Rives, Blandine Tramunt, Claire Naylies, Géraldine Garcia, Elodie Rousseau-Bacquié, Justine Bertrand-Michel, Cécile Canlet, Sylvie Chevolleau-Mege, Laurent Debrauwer, Christophe Heymes, Rémy Burcelin, Thierry Levade, Pierre Gourdy, Walter Wahli, Yuna Blum, Laurence Gamet-Payrastre, Sandrine Ellero-Simatos, Julie Guillermet-Guibert, Phillip Hawkins, Len Stephens, Catherine Postic, Alexandra Montagner, Nicolas Loiseau, Hervé Guillou

PLOS Biology: published April 14, 2025 | https://doi.org/10.1371/journal.pbio.3003112

A multi-strategy antimicrobial discovery approach reveals new ways to treat Chlamydia

Magnus Ölander, Daniel Rea Vázquez, Karsten Meier, Aakriti Singh, Amanda Silva de Sousa, Fabiola Puértolas-Balint, Milica Milivojevic, Lieke Mooij, Johanna Fredlund, Eduard Calpe Bosch, María Rayón Díaz, Moa Lundgren, Karin van der Wal, Shaochun Zhu, André Mateus, Bjoern O. Schroeder, Jeremy R. Lohman, Barbara S. Sixt

PLOS Biology: published April 29, 2025 | https://doi.org/10.1371/journal.pbio.3003123

Structure of the scaffolding protein and portal within the bacteriophage P22 procapsid provides insights into the self-assembly process

Hao Xiao, Wenyuan Chen, Hao Pang, Jing Zheng, Li Wang, Hao Feng, Jingdong Song, Lingpeng Cheng, Hongrong Liu

PLOS Biology: published April 17, 2025 | https://doi.org/10.1371/journal.pbio.3003104

Structures of G-protein coupled receptor HCAR1 in complex with Gi1 protein reveal the mechanistic basis for ligand recognition and agonist selectivity

Xin Pan, Fang Ye, Peiruo Ning, Yiping Yu, Zhiyi Zhang, Jingxuan Wang, Geng Chen, Zhangsong Wu, Chen Qiu, Jiancheng Li, Bangning Chen, Lizhe Zhu, Chungen Qian, Kaizheng Gong, Yang Du

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003126

Cross-species analysis of the nuclease Artemis highlights its evolving function in domesticating RAG-like transposons and residues that are crucial for activity

Ziwen Huang, Zhenxi Cai, Xin Tao, Xinli Wang, Xiaoxue Tian, Fan Chen, Zhen Li, Anlong Xu, Shaochun Yuan

PLOS Biology: published April 17, 2025 | https://doi.org/10.1371/journal.pbio.3003056

Short Reports

Neuronal responses in the human primary motor cortex coincide with the subjective onset of movement intention in brain–machine interface-mediated actions

Jean-Paul Noel, Marcie Bockbrader, Tommaso Bertoni, Sam Colachis, Marco Solca, Pavo Orepic, Patrick D. Ganzer, Patrick Haggard, Ali Rezai, Olaf Blanke, Andrea Serino

PLOS Biology: published April 17, 2025 | https://doi.org/10.1371/journal.pbio.3003118

Monocyte-secreted Wnt reduces the efficiency of central nervous system remyelination

Bianca M. Hill, Rebecca K. Holloway, Lindsey H. Forbes, Claire L. Davies, Jonathan K. Monteiro, Christina M. Brown, Jamie Rose, Neva Fudge, Pamela J. Plant, Ayisha Mahmood, Koroboshka Brand-Arzamendi, Sarah A. Kent, Irene Molina-Gonzalez, Stefka Gyoneva, Richard M. Ransohoff, Brian Wipke, Josef Priller, Raphael Schneider, Craig S. Moore, Veronique E. Miron

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003073

Normal locomotion in zebrafish lacking the sodium channel NaV1.4 suggests that the need for muscle action potentials is not universal

Chifumi Akiyama, Souhei Sakata, Fumihito Ono

PLOS Biology: published April 24, 2025 | https://doi.org/10.1371/journal.pbio.3003137

Activation of the Drosophila innate immune system accelerates growth in cooperation with oncogenic Ras

Fabienne Brutscher, Federico Germani, George Hausmann, Lena Jutz, Konrad Basler

PLOS Biology: published April 28, 2025 | https://doi.org/10.1371/journal.pbio.3003068

Membrane asymmetry facilitates murine norovirus entry and persistent enteric infection

Brittany M. Stewart, Linley R. Pierce, Mikayla C. Olson, Chengyuan Ji, Robert C. Orchard

PLOS Biology: published April 17, 2025 | https://doi.org/10.1371/journal.pbio.3003147

SGO2 does not play an essential role in separase inhibition during meiosis I in mouse oocytes

Benjamin Wetherall, David Bulmer, Alexandra Sarginson, Christopher Thomas, Suzanne Madgwick

PLOS Biology: published April 23, 2025 | https://doi.org/10.1371/journal.pbio.3003131

Membrane-associated σ factors disrupt rRNA operon clustering in Escherichia coli

Khang Ho, Rasika M. Harshey

PLOS Biology: published April 17, 2025 | https://doi.org/10.1371/journal.pbio.3003113

Mapping the ultrastructural topology of the corynebacterial cell surface

Buse Isbilir, Anna Yeates, Vikram Alva, Tanmay A. M. Bharat

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003130

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries

Jan Felix, Steven De Munck, J. Fernando Bazan, Savvas N. Savvides

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3003124

Methods and Resources

Scalable eQTL mapping using single-nucleus RNA-sequencing of recombined gametes from a small number of individuals

Matthew T. Parker, Samija Amar, José A. Campoy, Kristin Krause, Sergio Tusso, Magdalena Marek, Bruno Huettel, Korbinian Schneeberger

PLOS Biology: published April 25, 2025 | https://doi.org/10.1371/journal.pbio.3003085

Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses

Yeasmin Akter, Grace Jones, Grant J. Daskivich, Victoria Shifflett, Karina J. Vargas, Martin Hruska

PLOS Biology: published April 4, 2025 | https://doi.org/10.1371/journal.pbio.3002649

A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution

Shihao Zhou, Qiyu Zhu, Minho Eom, Shilin Fang, Oksana M. Subach, Chen Ran, Jonnathan Singh Alvarado, Praneel S. Sunkavalli, Yuanping Dong, Yangdong Wang, Jiewen Hu, Hanbin Zhang, Zhiyuan Wang, Xiaoting Sun, Tao Yang, Yu Mu, Young-Gyu Yoon, Zengcai V. Guo, Fedor V. Subach, Kiryl D. Piatkevich

PLOS Biology: published April 29, 2025 | https://doi.org/10.1371/journal.pbio.3003048

Expanding the bat toolbox: Carollia perspicillata bat cell lines and reagents enable the characterization of viral susceptibility and innate immune responses

Victoria Gonzalez, Cierra Word, Nahomi Guerra-Pilaquinga, Mitra Mazinani, Stephen Fawcett, Christine Portfors, Darryl Falzarano, Alison M. Kell, Rohit K. Jangra, Arinjay Banerjee, Stephanie N. Seifert, Michael Letko

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003098

Transcriptomic atlas throughout Coccidioides development reveals key phase-enriched transcripts of this important fungal pathogen

Christina M. Homer, Mark Voorhies, Keith Walcott, Elena Ochoa, Anita Sil

PLOS Biology: published April 15, 2025 | https://doi.org/10.1371/journal.pbio.3003066

MSP-tracker: A versatile vesicle tracking software tool used to reveal the spatial control of polarized secretion in Drosophila epithelial cells

Jennifer H. Richens, Mariia Dmitrieva, Helen L. Zenner, Nadine Muschalik, Richard Butler, Jade Glashauser, Carolina Camelo, Stefan Luschnig, Sean Munro, Jens Rittscher, Daniel St Johnston

PLOS Biology: published April 10, 2025 | https://doi.org/10.1371/journal.pbio.3003099

Completing the BASEL phage collection to unlock hidden diversity for systematic exploration of phage–host interactions

Dorentina Humolli, Damien Piel, Enea Maffei, Yannik Heyer, Elia Agustoni, Aisylu Shaidullina, Luc Willi, Patrick Imwinkelried, Fabienne Estermann, Aline Cuénod, Dominik P. Buser, Carola Alampi, Mohamed Chami, Adrian Egli, Sebastian Hiller, Matthew Dunne, Alexander Harms

PLOS Biology: published April 7, 2025 | https://doi.org/10.1371/journal.pbio.3003063

Extreme diversity of phage amplification rates and phage–antibiotic interactions revealed by PHORCE

Yuval Mulla, Janina Müller, Denny Trimcev, Tobias Bollenbach

PLOS Biology: published April 8, 2025 | https://doi.org/10.1371/journal.pbio.3003065

Meta-Research Article

Testing the reproducibility of ecological studies on insect behavior in a multi-laboratory setting identifies opportunities for improving experimental rigor

Carolin Mundinger, Nora K. E. Schulz, Pragya Singh, Steven Janz, Maximilian Schurig, Jacob Seidemann, Joachim Kurtz, Caroline Müller, Holger Schielzeth, Vanessa T. von Kortzfleisch, S. Helene Richter

PLOS Biology: published April 22, 2025 | https://doi.org/10.1371/journal.pbio.3003019

Preregistered Research Article

Removing facial features from structural MRI images biases visual quality assessment

Céline Provins, Élodie Savary, Thomas Sanchez, Emeline Mullier, Jaime Barranco, Elda Fischi-Gómez, Yasser Alemán-Gómez, Jonas Richiardi, Russell A. Poldrack, Patric Hagmann, Oscar Esteban

PLOS Biology: published April 30, 2025 | https://doi.org/10.1371/journal.pbio.3003149

Discovery Report

The SPFH complex HflK-HflC regulates aerobic respiration in bacteria

María Isabel Pérez-López, Paul Lubrano, Georgia Angelidou, Sarah Hoch, Timo Glatter, Nicole Paczia, Hannes Link, Victor Sourjik

PLOS Biology: published April 7, 2025 | https://doi.org/10.1371/journal.pbio.3003077

Update Article

Social knowledge about others is anchored to self-knowledge in the hippocampal formation

Marta Rodríguez Aramendía, Mariachiara Esposito, Raphael Kaplan

PLOS Biology: published April 2, 2025 | https://doi.org/10.1371/journal.pbio.3003050

This page is brought to you by Brian Golding (Golding@McMaster.CA) and is copied locally here to speed your access. To go to the original page (should you find something interesting or should you wish to follow links) click on Current Issue of PLoSBiology

April 2025

Cover

Completing the BASEL phage collection to unlock hidden diversity for systematic exploration of phage–host interactions

Editorial

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Unsolved Mystery

Perspectives

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Primers

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Research Articles

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Short Reports

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Methods and Resources

Meta-Research Article

Preregistered Research Article

Discovery Report

Update Article

Related Articles

This page is brought to you by Brian Golding (Golding@McMaster.CA) and is copied locally here to speed your access. To go to the original page (should you find something interesting or should you wish to follow links) click on

Current Issue of PLoSBiology