An agent-based model of electrochemical signaling in a Bacillus subtilis biofilm predicts spatially patterned membrane potential oscillations (left side), which are validated in experiments with fluorescent membrane potential reporters in microfluidic-grown biofilms (right side). The line traces show out-of-phase signaling dynamics between the biofilm interior and exterior, found in both our model and in vitro observations. Mulder et al. 2025
Image Credit: Maya Peters Kostman
Education Articles
Ten simple rules for effective research data management
PLOS Computational Biology: published December 8, 2025 | https://doi.org/10.1371/journal.pcbi.1013779
Eleven quick tips for organizing a data cleaning challenge
PLOS Computational Biology: published December 16, 2025 | https://doi.org/10.1371/journal.pcbi.1013791
Ten simple rules for running a virtual program to introduce computational biology at the high school level
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013830
Ten simple rules on how to set up an Innovation Boot Camp for exploitation of research results
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013807
Review
Computational modeling of rhythmic expectations: Perspectives, pitfalls, and prospects
PLOS Computational Biology: published December 10, 2025 | https://doi.org/10.1371/journal.pcbi.1013798
Research Articles
Integrative multi-omics framework for causal gene discovery in Long COVID
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013725
DDGWizard: Integration of feature calculation resources for analysis and prediction of changes in protein thermostability upon point mutations
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013783
Nucleotide context models outperform protein language models for predicting antibody affinity maturation
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013758
Long-read sequencing transcriptome quantification with lr-kallisto
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013692
Using evolutionary context to classify difficult protein folds
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013773
D3Impute: Dropout-aware discrimination, distribution-aware modeling, and density-guide imputation for scRNA-seq data
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013744
Attenuation of HIV severity by slightly deleterious mutations can explain the long-term trajectory of virulence evolution
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013131
A unified model library maps how neuromodulation reshapes the excitability landscape of neurons across the brain
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013765
Neural mechanisms of flexible perceptual inference
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013675
Automated registration and clustering for enhanced localization atomic force microscopy of flexible membrane proteins
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013277
Identification of dynamic models of microbial communities: A workflow addressing identifiability and modeling pitfalls
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013204
Do we advise as one likes? The alignment bias in social advice giving
PLOS Computational Biology: published December 2, 2025 | https://doi.org/10.1371/journal.pcbi.1013732
Unsupervised detection and fitness estimation of emerging SARS-CoV-2 variants: Application to wastewater samples (ANRS0160)
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1013749
Natural variants of von Willebrand factor R1205 causing von Willebrand disease with accelerated von Willebrand factor clearance: In silico docking models and energetics of the interaction with both LRP1 and GpIb A1 domain
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1013458
Accuracy of the Lotka-Volterra model fails in strongly coupled microbial consumer-resource systems
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1013719
Epigenetics is all you need: A transformer to decode chromatin structural compartments from the epigenome
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1012326
Ohno-miRNAs: miRNA pairs derived from whole-genome duplication
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1013766
Receptor dimerization enables ligand discrimination through tunable response heterogeneity
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1013781
Decoupled contrastive multi-view clustering with adaptive false negative elimination for cancer subtyping
PLOS Computational Biology: published December 4, 2025 | https://doi.org/10.1371/journal.pcbi.1013780
Coexistence rules for small, antagonistically interacting microbial communities
PLOS Computational Biology: published December 4, 2025 | https://doi.org/10.1371/journal.pcbi.1013763
Random time-shift approximation enables hierarchical Bayesian inference of mechanistic within-host viral dynamics models on large datasets
PLOS Computational Biology: published December 4, 2025 | https://doi.org/10.1371/journal.pcbi.1013775
ARTreeFormer: A faster attention-based autoregressive model for phylogenetic inference
PLOS Computational Biology: published December 4, 2025 | https://doi.org/10.1371/journal.pcbi.1013768
An agent-based model of metabolic signaling oscillations in Bacillus subtilis biofilms
PLOS Computational Biology: published December 4, 2025 | https://doi.org/10.1371/journal.pcbi.1013746
Verification and reproducible curation of the BioModels repository
PLOS Computational Biology: published December 4, 2025 | https://doi.org/10.1371/journal.pcbi.1013239
Benchmarking interpretability of deep learning for predictive genomics: Recall, precision, and variability of feature attribution
PLOS Computational Biology: published December 5, 2025 | https://doi.org/10.1371/journal.pcbi.1013784
Systematic computational assessment of atrial function impairment due to fibrotic remodeling in electromechanical properties
PLOS Computational Biology: published December 5, 2025 | https://doi.org/10.1371/journal.pcbi.1013265
Graph-enhanced deep learning for diabetic retinopathy diagnosis: A quality-aware and uncertainty-driven approach
PLOS Computational Biology: published December 5, 2025 | https://doi.org/10.1371/journal.pcbi.1013745
Memory, innovation and vertical learning
PLOS Computational Biology: published December 5, 2025 | https://doi.org/10.1371/journal.pcbi.1013785
Transient boosting of action potential backpropagation for few-shot temporal pattern learning
PLOS Computational Biology: published December 5, 2025 | https://doi.org/10.1371/journal.pcbi.1013777
A meta-contrastive learning approach for clinical drug-drug interaction extraction from biomedical literature
PLOS Computational Biology: published December 5, 2025 | https://doi.org/10.1371/journal.pcbi.1013722
Observing hidden neuronal states in experiments
PLOS Computational Biology: published December 8, 2025 | https://doi.org/10.1371/journal.pcbi.1013748
Whom do we prefer to learn from in observational reinforcement learning?
PLOS Computational Biology: published December 8, 2025 | https://doi.org/10.1371/journal.pcbi.1013143
Neural encoding of biomechanically (im)possible human movements in occipitotemporal cortex
PLOS Computational Biology: published December 8, 2025 | https://doi.org/10.1371/journal.pcbi.1013694
Learning structured population models from data with WSINDy
PLOS Computational Biology: published December 8, 2025 | https://doi.org/10.1371/journal.pcbi.1013742
Dynamical stability and mechanical impedance are optimized when manipulating uncertain dynamically complex objects
PLOS Computational Biology: published December 9, 2025 | https://doi.org/10.1371/journal.pcbi.1013797
Comparing a computational model of visual problem solving with human vision on a difficult vision task
PLOS Computational Biology: published December 9, 2025 | https://doi.org/10.1371/journal.pcbi.1012968
Randomized Spatial PCA (RASP): A computationally efficient method for dimensionality reduction of high-resolution spatial transcriptomics data
PLOS Computational Biology: published December 10, 2025 | https://doi.org/10.1371/journal.pcbi.1013759
Modeling the interplay of sex hormones in cardiac hypertrophic signaling
PLOS Computational Biology: published December 10, 2025 | https://doi.org/10.1371/journal.pcbi.1012895
Computational characterization of the xanthan gum glycosyltransferase GumK
PLOS Computational Biology: published December 10, 2025 | https://doi.org/10.1371/journal.pcbi.1013803
How cells tame noise while maintaining ultrasensitive transcriptional responses
PLOS Computational Biology: published December 11, 2025 | https://doi.org/10.1371/journal.pcbi.1013217
Axon collateralization and focal myelin dystrophy alter action potential propagation in multicompartment pyramidal neuron models
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013733
Lysergic acid diethylamide-derived excitatory/inhibitory ratio change enhances global synchrony in functional brain dynamics
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013822
Semi-supervised Bayesian integration of multiple spatial proteomics datasets
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013799
Computational origins of shape perception
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013674
Predicting neural responses to intra- and extra-cranial electric brain stimulation by means of the reciprocity theorem
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013782
Sketch, capture and layout phylogenies
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013805
Assessing the relative contributions of mosaic and regulatory developmental modes from single-cell trajectories
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1012352
The dynamics of explore-exploit decisions suggest a threshold mechanism for reduced random exploration in older adults
PLOS Computational Biology: published December 16, 2025 | https://doi.org/10.1371/journal.pcbi.1012873
Blaming luck, claiming skill: Self-attribution bias in error assignment
PLOS Computational Biology: published December 16, 2025 | https://doi.org/10.1371/journal.pcbi.1013787
Probabilistic modeling of cell cycle dynamics in response to cell cycle targeting chemotherapy drugs to guide treatment strategies
PLOS Computational Biology: published December 16, 2025 | https://doi.org/10.1371/journal.pcbi.1013790
Quantifying viral pandemic potential from experimental transmission studies
PLOS Computational Biology: published December 17, 2025 | https://doi.org/10.1371/journal.pcbi.1013808
Quantifying vector diversion effects in zoonotic systems: A modelling framework for arbovirus transmission between reservoir and dead-end hosts
PLOS Computational Biology: published December 18, 2025 | https://doi.org/10.1371/journal.pcbi.1013359
Mechanistically informed machine learning links non-canonical TCA cycle activity to Warburg metabolism and hallmarks of malignancy
PLOS Computational Biology: published December 18, 2025 | https://doi.org/10.1371/journal.pcbi.1013384
Decoding the role of DNA sequence on protein-DNA co-condensation
PLOS Computational Biology: published December 18, 2025 | https://doi.org/10.1371/journal.pcbi.1013829
ASPEN: Robust detection of allelic dynamics in single cell RNA-seq
PLOS Computational Biology: published December 19, 2025 | https://doi.org/10.1371/journal.pcbi.1013837
Stable individualized brain computing model informed by spatiotemporal co-activity patterns
PLOS Computational Biology: published December 19, 2025 | https://doi.org/10.1371/journal.pcbi.1013817
Zero-shot deep learning for the annotation of unknown eDNA sequences using co-occurrences and phylogenetic embeddings
PLOS Computational Biology: published December 19, 2025 | https://doi.org/10.1371/journal.pcbi.1013776
DAGFormer: A graph-based domain adaptation approach for single-cell cancer drug response prediction
PLOS Computational Biology: published December 19, 2025 | https://doi.org/10.1371/journal.pcbi.1013832
Long-term perceptual priors drive confidence bias that favors prior-congruent evidence
PLOS Computational Biology: published December 22, 2025 | https://doi.org/10.1371/journal.pcbi.1013826
Abnormal vasculature reduces overlap between drugs and oxygen in a tumour computational model: Implications for therapeutic efficacy
PLOS Computational Biology: published December 22, 2025 | https://doi.org/10.1371/journal.pcbi.1013801
Neuronal identity is not static: An input-driven perspective
PLOS Computational Biology: published December 22, 2025 | https://doi.org/10.1371/journal.pcbi.1013821
V-Cornea: A computational model of corneal epithelium homeostasis, injury, and recovery
PLOS Computational Biology: published December 26, 2025 | https://doi.org/10.1371/journal.pcbi.1013410
A game theoretic treatment of contagion in trade networks
PLOS Computational Biology: published December 26, 2025 | https://doi.org/10.1371/journal.pcbi.1013845
Interplay of structured and random interactions in complex ecosystems dynamics
PLOS Computational Biology: published December 26, 2025 | https://doi.org/10.1371/journal.pcbi.1013786
Modeling based insights into mechanical dysfunction in esophageal motility disorders
PLOS Computational Biology: published December 26, 2025 | https://doi.org/10.1371/journal.pcbi.1013778
A novel generative framework for designing pathogen-targeted antimicrobial peptides with programmable physicochemical properties
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013833
Cell-DINO: Self-supervised image-based embeddings for cell fluorescent microscopy
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013828
Ion dynamics underlying the seizure delay effect of low-frequency electrical stimulation
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013838
Modeling tissue-scale ciliary transport and mixing in three-dimensional Newtonian flow
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1012766
A data-driven biology-based network model reproduces C. elegans premotor neural dynamics
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013818
Using artificial neural networks to reveal the human confidence computation
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013827
A computational framework to study EGFR signaling distribution in egg chambers during dynamic interactions between soma and germline
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013802
Gaussian process emulation for exploring complex infectious disease models
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013849
Inverse modeling unveils governing law of mechano-chemical dynamics of epithelial migration
PLOS Computational Biology: published December 29, 2025 | https://doi.org/10.1371/journal.pcbi.1013854
Coancestry superposed on admixed populations yields measures of relatedness at individual-level resolution
PLOS Computational Biology: published December 31, 2025 | https://doi.org/10.1371/journal.pcbi.1013848
Correction
Correction: Data-driven modeling predicts gene regulatory network dynamics during the differentiation of multipotential hematopoietic progenitors
PLOS Computational Biology: published December 19, 2025 | https://doi.org/10.1371/journal.pcbi.1013823
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Formal Comment
Comment on “Using genomic data and machine learning to predict antibiotic resistance: A tutorial paper”
PLOS Computational Biology: published December 1, 2025 | https://doi.org/10.1371/journal.pcbi.1013673
Methods
A deep learning approach for time-consistent cell cycle phase prediction from microscopy data
PLOS Computational Biology: published December 11, 2025 | https://doi.org/10.1371/journal.pcbi.1013800
MAGNET: Multi-view graph autoencoder with cell-gene attention for cell interaction network reconstruction from spatial transcriptomics
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013810
When predict can also explain: Few-shot prediction to select better neural latents
PLOS Computational Biology: published December 30, 2025 | https://doi.org/10.1371/journal.pcbi.1013789
Software
BAGEL: Protein engineering via exploration of an energy landscape
PLOS Computational Biology: published December 3, 2025 | https://doi.org/10.1371/journal.pcbi.1013774
PyFibers: An open-source NEURON-Python package to simulate responses of model nerve fibers to electrical stimulation
PLOS Computational Biology: published December 12, 2025 | https://doi.org/10.1371/journal.pcbi.1013764
LCMSpector: A simple open-source viewer for targeted hyphenated mass spectrometry analysis
PLOS Computational Biology: published December 15, 2025 | https://doi.org/10.1371/journal.pcbi.1013095
A methodology to reduce the localization error in multi-loci microscopy provides new insights into enhancer biology
PLOS Computational Biology: published December 17, 2025 | https://doi.org/10.1371/journal.pcbi.1013796
Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud
PLOS Computational Biology: published December 19, 2025 | https://doi.org/10.1371/journal.pcbi.1013819