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Current Issue of PLoSBiology


Current Issue | PLOS Computational Biology
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December 2025

An agent-based model of electrochemical signaling in a Bacillus subtilis biofilm predicts spatially patterned membrane potential oscillations (left side), which are validated in experiments with fluorescent membrane potential reporters in microfluidic-grown biofilms (right side). The line traces show out-of-phase signaling dynamics between the biofilm interior and exterior, found in both our model and in vitro observations. Mulder et al. 2025

Image Credit: Maya Peters Kostman

Education Articles

Ten simple rules for effective research data management

Max J. Hassenstein, Klaus Jung

Eleven quick tips for organizing a data cleaning challenge

Ireen J. Kal, Lidwien P. Smabers, Joanna von Berg, Giulia Perticari, Mette van de Meent, Denise Fokkelman, Joep Sprangers, Loïc Lannelongue, Florijn Dekkers

Ten simple rules for running a virtual program to introduce computational biology at the high school level

Hanako Osuga, Matthew C. Chan, Katherine Brower, Liza J. Ray, Jeanne T. Chowning

Ten simple rules on how to set up an Innovation Boot Camp for exploitation of research results

Susanne Hollmann, Christiane Hollmann, Lesley Tobin de Fuentes, Sady Roberto Rodriguez, Yerko Fredes, Antonio Garcia-Moyano, Christina Rørvik, Babette Regierer

Review

Computational modeling of rhythmic expectations: Perspectives, pitfalls, and prospects

Atser Damsma, Jonathan Cannon, Lauren K. Fink, Keith B. Doelling, Jessica A. Grahn, Henkjan Honing, Thomas Kaplan, Edward W. Large, Fleur L. Bouwer

Research Articles

Integrative multi-omics framework for causal gene discovery in Long COVID

Sindy Pinero, Xiaomei Li, Lin Liu, Jiuyong Li, Sang Hong Lee, Marnie Winter, Thin Nguyen, Junpeng Zhang, Thuc Duy Le

DDGWizard: Integration of feature calculation resources for analysis and prediction of changes in protein thermostability upon point mutations

Mingkai Wang, Khaled Jumah, Qun Shao, Katarzyna Kamieniecka, Yihan Liu, Krzysztof Poterlowicz

Nucleotide context models outperform protein language models for predicting antibody affinity maturation

Mackenzie M. Johnson, Kevin Sung, Hugh K. Haddox, Ashni A. Vora, Tatsuya Araki, Gabriel D. Victora, Yun S. Song, Julia Fukuyama, Frederick A. Matsen IV

Long-read sequencing transcriptome quantification with lr-kallisto

Rebekah K. Loving, Delaney K. Sullivan, Fairlie Reese, Elisabeth Rebboah, Jasmine Sakr, Narges Rezaie, Heidi Y. Liang, Ghassan Filimban, Shimako Kawauchi, A. Sina Booeshaghi, Páll Melsted, Conrad Oakes, Diane Trout, Brian A. Williams, Grant R. MacGregor, Barbara J. Wold, Ali Mortazavi, Lior Pachter

Using evolutionary context to classify difficult protein folds

Jimin Pei, R. Dustin Schaeffer, Qian Cong, Nick V. Grishin

Attenuation of HIV severity by slightly deleterious mutations can explain the long-term trajectory of virulence evolution

Harriet Longley, Christophe Fraser, Chris Wymant, Katrina Lythgoe

A unified model library maps how neuromodulation reshapes the excitability landscape of neurons across the brain

Domenico Guarino, Ilaria Carannante, Alain Destexhe

Neural mechanisms of flexible perceptual inference

John Schwarcz, Jan Bauer, Haneen Rajabi, Gabrielle Marmur, Robert Reiner, Eran Lottem, Jonathan Kadmon

Automated registration and clustering for enhanced localization atomic force microscopy of flexible membrane proteins

Creighton M. Lisowski, Gavin M. King, Ioan Kosztin

Identification of dynamic models of microbial communities: A workflow addressing identifiability and modeling pitfalls

Ana Paredes-Vázquez, Eva Balsa-Canto, Julio R. Banga

Do we advise as one likes? The alignment bias in social advice giving

Xitong Luo, Lei Zhang, Yafeng Pan

Unsupervised detection and fitness estimation of emerging SARS-CoV-2 variants: Application to wastewater samples (ANRS0160)

Alexandra Lefebvre, Vincent Maréchal, Arnaud Gloaguen, The Obépine Consortium, Amaury Lambert, Yvon Maday

Natural variants of von Willebrand factor R1205 causing von Willebrand disease with accelerated von Willebrand factor clearance: In silico docking models and energetics of the interaction with both LRP1 and GpIb A1 domain

Monica Sacco, Stefano Lancellotti, Antonietta Ferretti, Maria Basso, Leonardo Di Gennaro, Giancarlo Castaman, Raimondo De Cristofaro

Accuracy of the Lotka-Volterra model fails in strongly coupled microbial consumer-resource systems

Michael P. Mustri, Quqiming Duan, Samraat Pawar

Epigenetics is all you need: A transformer to decode chromatin structural compartments from the epigenome

Esteban Dodero-Rojas, Angel Mendieta, Yao Fehlis, Nicholas Mayala, Vinícius G. Contessoto, José N. Onuchic

Ohno-miRNAs: miRNA pairs derived from whole-genome duplication

Leonardo Agasso, Ivan Molineris, Michele Caselle

Decoupled contrastive multi-view clustering with adaptive false negative elimination for cancer subtyping

Mengxiang Lin, Rongqi Fan, Saisai Zhu, Xiaoqiang Yan, Quan Zou, Zhen Tian

Coexistence rules for small, antagonistically interacting microbial communities

Gaurav S. Athreya, Chaitanya S. Gokhale, Prateek Verma

ARTreeFormer: A faster attention-based autoregressive model for phylogenetic inference

Tianyu Xie, Yicong Mao, Cheng Zhang

An agent-based model of metabolic signaling oscillations in Bacillus subtilis biofilms

Obadiah J. Mulder, Maya Peters Kostman, Abdulrahmen Almodaimegh, Michael D. Edge, Joseph W. Larkin

Verification and reproducible curation of the BioModels repository

Lucian P. Smith, Rahuman S. Malik-Sheriff, Tung V. N. Nguyen, Henning Hermjakob, Jonathan Karr, Bilal Shaikh, Logan Drescher, Ion I. Moraru, James C. Schaff, Eran Agmon, Alexander A. Patrie, Michael L. Blinov, Joseph L. Hellerstein, Elebeoba E. May, David P. Nickerson, John H. Gennari, Herbert M. Sauro

Systematic computational assessment of atrial function impairment due to fibrotic remodeling in electromechanical properties

Åshild Telle, Ahmad Kassar, Nadia Chamoun, Romanos Haykal, Alejandro Gonzalo, Tori Hensley, Yaacoub Chahine, Oscar Flores, Juan C. del Álamo, Nazem Akoum, Christoph M. Augustin, Patrick M. Boyle

Graph-enhanced deep learning for diabetic retinopathy diagnosis: A quality-aware and uncertainty-driven approach

Zarin Akter, Jawad Ibn Ahad, Md. Mutasim Farhan, Riasat Khan

Memory, innovation and vertical learning

Madeleine Ammar, Laurel Fogarty, Anne Kandler

Observing hidden neuronal states in experiments

Dmitry Amakhin, Anton Chizhov, Guillaume Girier, Mathieu Desroches, Jan Sieber, Serafim Rodrigues

Whom do we prefer to learn from in observational reinforcement learning?

Gota Morishita, Carsten Murawski, Nitin Yadav, Shinsuke Suzuki

Neural encoding of biomechanically (im)possible human movements in occipitotemporal cortex

Giuseppe Marrazzo, Federico De Martino, Albert Mukovskiy, Martin A. Giese, Beatrice de Gelder

Learning structured population models from data with WSINDy

Rainey Lyons, Vanja Dukic, David M. Bortz

Modeling the interplay of sex hormones in cardiac hypertrophic signaling

Adhithi Lakshmikanthan, Minnie Kay, Pim J.A. Oomen

Computational characterization of the xanthan gum glycosyltransferase GumK

Davide Luciano, F. Emil Thomasen, Kresten Lindorff-Larsen, Gaston Courtade

How cells tame noise while maintaining ultrasensitive transcriptional responses

Eui Min Jeong, Chang Yoon Chung, Jae Kyoung Kim

Axon collateralization and focal myelin dystrophy alter action potential propagation in multicompartment pyramidal neuron models

Nilapratim Sengupta, Jennifer I. Luebke, Christina M. Weaver

Lysergic acid diethylamide-derived excitatory/inhibitory ratio change enhances global synchrony in functional brain dynamics

Lingyu Zhang, Weiyang Shi, Ziyang Zhao, Zhichao Wang, Congying Chu, Bokai Zhao, Jiaqi Zhang, Qianhui Liu, Yueheng Lan, Tianzi Jiang

Semi-supervised Bayesian integration of multiple spatial proteomics datasets

Stephen Coleman, Lisa Breckels, Ross F. Waller, Kathryn S. Lilley, Chris Wallace, Oliver M. Crook, Paul D. W. Kirk

Computational origins of shape perception

Lalit Pandey, Samantha M. W. Wood, Justin N. Wood

Predicting neural responses to intra- and extra-cranial electric brain stimulation by means of the reciprocity theorem

Torbjørn V. Ness, Christof Koch, Gaute T. Einevoll

Sketch, capture and layout phylogenies

Daniel H. Huson

The dynamics of explore-exploit decisions suggest a threshold mechanism for reduced random exploration in older adults

Caroline E. Phelps, Alec E. Frisvold, Meghna Sreeram, Victoria D. Antoniou, Larissa E. Oliveira, Sierra R. Tooke, Yan Z. Lu, Anna C. Lemon, Joseph U. Fraire, Alexe G. Delval, Betsy K. Smith, Devynn H. Spangenberg, Madelien N. Mithelavage, Michelle N. Ngo, Elizabeth M. O. Keller, Liliana J. Issac, Sara A. Harader, Jack-Morgan Mizell, Siyu Wang, Waitsang Keung, Mark H. Sundman, Mary-Kathryn Franchetti, Ying-hui Chou, Gene E. Alexander, Robert C. Wilson

Blaming luck, claiming skill: Self-attribution bias in error assignment

Naoyuki Okamoto, Michael Taylor, Takatomi Kubo, Shin Ishii, Benedetto De Martino, Aurelio Cortese

Quantifying viral pandemic potential from experimental transmission studies

Elizabeth D. Somsen, Kayla M. Septer, Cassandra J. Field, Devanshi R. Patel, Anice C. Lowen, Troy C. Sutton, Katia Koelle

Quantifying vector diversion effects in zoonotic systems: A modelling framework for arbovirus transmission between reservoir and dead-end hosts

Emma L. Fairbanks, Matthew Baylis, Janet M. Daly, Michael J. Tildesley

Mechanistically informed machine learning links non-canonical TCA cycle activity to Warburg metabolism and hallmarks of malignancy

Lihao Lin, Francesco Lapi, Bruno Giovanni Galuzzi, Marco Vanoni, Lilia Alberghina, Chiara Damiani

Decoding the role of DNA sequence on protein-DNA co-condensation

Rohit Kumar Singh, Pinaki Swain, Mahipal Ganji, Sandeep Choubey

ASPEN: Robust detection of allelic dynamics in single cell RNA-seq

Veronika Petrova, Muqing Niu, Thomas S. Vierbuchen, Emily S. Wong

Stable individualized brain computing model informed by spatiotemporal co-activity patterns

Lan Yang, Jiayu Lu, Xinran Wu, Xi Zhang, Ting Li, Ruiyun Chang, Songjun Peng, Dandan Li, Jie Zhang, Bin Wang

Zero-shot deep learning for the annotation of unknown eDNA sequences using co-occurrences and phylogenetic embeddings

Steven Stalder, Théophile Sanchez, Michele Volpi, Stéphanie Manel, David Mouillot, Arnaud Auber, Morgane Bruno, Virginie Marques, Camille Albouy, Loïc Pellissier

Long-term perceptual priors drive confidence bias that favors prior-congruent evidence

Marika Constant, Elisa Filevich, Pascal Mamassian

Abnormal vasculature reduces overlap between drugs and oxygen in a tumour computational model: Implications for therapeutic efficacy

Romain Enjalbert, Jakub Köry, Timm Krüger, Miguel O. Bernabeu

Neuronal identity is not static: An input-driven perspective

Nishant Joshi, Sven van Der Burg, Tansu Celikel, Fleur Zeldenrust

V-Cornea: A computational model of corneal epithelium homeostasis, injury, and recovery

Joel Vanin, Michael Getz, Catherine Mahony, Thomas B. Knudsen, James A. Glazier

A game theoretic treatment of contagion in trade networks

John S. McAlister, Jesse L. Brunner, Danielle J. Galvin, Nina H. Fefferman

Interplay of structured and random interactions in complex ecosystems dynamics

Juan Giral Martínez, Matthieu Barbier, Silvia De Monte

Modeling based insights into mechanical dysfunction in esophageal motility disorders

Guy Elisha, Sourav Halder, Xinyi Liu, Dustin A. Carlson, Peter J. Kahrilas, John E. Pandolfino, Neelesh A. Patankar

A novel generative framework for designing pathogen-targeted antimicrobial peptides with programmable physicochemical properties

Weizhong Zhao, Kaijieyi Hou, Chang Tang, Yiting Shen, Jinlin Liu, Xiaohua Hu

Cell-DINO: Self-supervised image-based embeddings for cell fluorescent microscopy

Théo Moutakanni, Camille Couprie, Seungeun Yi, Michael Doron, Zitong S. Chen, Nikita Moshkov, Elouan Gardes, Mathilde Caron, Hugo Touvron, Armand Joulin, Piotr Bojanowski, Wolfgang M. Pernice, Juan C. Caicedo

Ion dynamics underlying the seizure delay effect of low-frequency electrical stimulation

Guillaume Girier, Isa Dallmer-Zerbe, Jan Chvojka, Jan Kudlacek, Premysl Jiruska, Jaroslav Hlinka, Helmut Schmidt

Modeling tissue-scale ciliary transport and mixing in three-dimensional Newtonian flow

Ling Xu, Pejman Senaei, Yi Jiang

A data-driven biology-based network model reproduces C. elegans premotor neural dynamics

Megan Morrison, Lai-Sang Young

Using artificial neural networks to reveal the human confidence computation

Medha Shekhar, Herrick Fung, Krish Saxena, Farshad Rafiei, Dobromir Rahnev

A computational framework to study EGFR signaling distribution in egg chambers during dynamic interactions between soma and germline

Nastassia Pouradier Duteil, Nicole T. Revaitis, Mathew G. Niepielko, Eric A. Klein, Nir Yakoby, Benedetto Piccoli

Gaussian process emulation for exploring complex infectious disease models

Anna M. Langmüller, Kiran A. Chandrasekher, Benjamin C. Haller, Samuel E. Champer, Courtney C. Murdock, Philipp W. Messer

Inverse modeling unveils governing law of mechano-chemical dynamics of epithelial migration

Yuto Kikuchi, Yoshifumi Asakura, Kazuhiro Aoki, Yohei Kondo, Honda Naoki

Formal Comment

Methods

A deep learning approach for time-consistent cell cycle phase prediction from microscopy data

Thomas Bonte, Oriane Pourcelot, Adham Safieddine, Floric Slimani, Florian Mueller, Dominique Weil, Edouard Bertrand, Thomas Walter

When predict can also explain: Few-shot prediction to select better neural latents

Kabir V. Dabholkar, Omri Barak

Software

BAGEL: Protein engineering via exploration of an energy landscape

Jakub Lála, Ayham Al-Saffar, Stefano Angioletti-Uberti

PyFibers: An open-source NEURON-Python package to simulate responses of model nerve fibers to electrical stimulation

Daniel P. Marshall, Elie S. Farah, Eric D. Musselman, Nicole A. Pelot, Warren M. Grill

LCMSpector: A simple open-source viewer for targeted hyphenated mass spectrometry analysis

Mateusz Fido, Etienne Hoesli, Elisa Cappio Barazzone, Renato Zenobi, Emma Slack

Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud

Jacob Bradford, Divya Joy, Mattias Winsen, Nicholas Meurant, Mackenzie Wilkins, Laurence O.W. Wilson, Denis C. Bauer, Dimitri Perrin