Hi all, here's a compilation of responses to my question about 454 sequencing facilities. cheers, Ryan Original post: can anyone recommend a US core facility for 454 sequencing? We want to do one full run using GS FLX (non-titanium) long-read chemistry (= 250bp av. reads), and the sstDNA library prep has already been done... so just emPCR titration & shotgun sequencing for this particular project. Main considerations would be (1) cost, and (2) turn-around time. Any suggestions would be much appreciated. General outcome: (1) Seem to be looking at $US10-12K for a full plate run (incl. titration), but library prep will add another $500+. (2) minimum 10-14 days, up to 4 weeks in some cases. If have want to do just a 1/2 plate or less, will need to wait for someone else with similarly-sized project, so that the plate can be shared (3) worth noting that Roche/454 are rapidly phasing out the non-titanium option, so regaents will become harder to come by soon. This also means that if you want to run only a 1/2 plate with this older chemistry, you might end up waiting a long time for a partner to share that other 1/2 of the plate with you. Interesting website: Google map of sequencing facilities in the URL below and you can do limited searches for 454 sequencers (tick box on the right): http://pathogenomics.bham.ac.uk/hts/ Non-exhaustive list (thanks to all for suggestions!): * Note that prices, where given, are not necessarily directly comparable. I apologise for any errors that I may have made. EnGenCore (recommended by a number of people, incl. Roche/454 Life Sciences) University of South Carolina Columbia, SC 29208, USA http://engencore.sc.edu/ * US11.7K full plate VBI Core Lab Virginia Bioinformatics Institute Washington Street Blacksburg, VA 24061, USA http://www.vbi.vt.edu/core/ * US11.8K full plate Macrogen Korea http://www.macrogen.co.kr/eng/ngs/ngs_main.jsp * US$12K full plate, first library prep free, further preps US$500 454 Life Sciences Branford, CT 06405, USA http://454.com/contact-us/request-sequencing-services.asp * US20K full plate (user must NOT do library prep) Genome Sequencing Laboratory New Mexico State University http://biology-web.nmsu.edu/houde/conspirators.htm * US11K full plate Emory GRA Genome Center, Emory University School of Medicine 201 Dowman Drive. Atlanta, Georgia 30322 USA http://www.corelabs.emory.edu/labs/gra_genome_center/index.html http://www.corelabs.emory.edu/labs/gra_genome_center/rates.html * US10.2K full plate Purdue Genomics Core Facility Purdue University West Lafayette, IN 47907 http://www.genomics.purdue.edu/~core/ * US15K full plate High-Throughput Sequencing and Genotyping Unit University of Illinois 1201 W. Gregory Drive, Urbana, IL 61801 http://www.biotech.uiuc.edu/centers/Keck/Highthroughput/ * US9.4K full plate Institute for Genome Sciences & Policy (IGSP) Duke University CIEMAS Durham, NC 27708, USA http://www.genome.duke.edu/cores/sequencing Indiana University, Center for Genomics and Bioinformatics http://cgb.indiana.edu/services/genome_sequencing/ IBEST DNA Sequencing Analysis Core University of Idaho Life Science South, Moscow, Idaho 83843 http://www.ibest.uidaho.edu/home/research_facilities.php Genome sequencing core University of Virginia Charlottesville, VA. 22904 http://faculty.virginia.edu/454seq/ Institute for Genome Sciences University of Maryland School of Medicine http://www.igs.umaryland.edu/454.php ryan.garrick@yale.edu