Dear EvolDir Folks, I received just a few responses to my questions about cost-saving tips for sequencing with an ABI 3100 (see original post at bottom). Here are the main points: * It is possible to buy generic polymer from MCLab (www.mclab.com), who produces their own versions of POP4-7. * It is possible to get a service contract from the third party company SeqGen (www.seqgen.net). * Labs seem to commonly use capillaries for 600-700 runs. * One person suggested that the life an array can be extended to 1000 runs by not cleaning the polymer blocks unless the array is being changed. The idea is that limiting the array's exposure to water particles helps it last longer. * I did not hear from anyone using POP7 with an ABI 3100. Thanks for the responses, -Jeff ORIGINAL POST: Our department has an ABI 3100 sequencer. This machine uses a 16 capillary array, the older syringe system, and POP6 polymer. We are currently in a situation where most labs send sequences out to core facilities, but some people still use the machine for small batches of samples. Therefore, we're exploring ways to cost effectively run the machine given the limited throughput and increases in ABI pricing. I assume that many labs are in a similar situation, and I was looking for some input on how people are dealing with common issues: Polymer -ABI is now only selling POP6 in 7 mL aliquots (list price: $468). Does anyone order POP6 from a different distributor at a better price? -POP7 can be bought in bulk, and although it is thinner it seems that people have used it in a 3100 (see link below). I'm curious to hear about experiences with this, particularly regarding syringe leakage. http://www.biotechniques.com/multimedia/archive/00036/BTN_A_04366BM01_- O_36600a.pdf Capillary arrays and regeneration: We currently use The Gel Company's array regeneration service ($200), usually sending arrays out every 6 months or 200 runs. I'd be interested in hearing about experiences with number of runs between regenerations, lifetime of arrays, other companies, or doing this yourself. Any other general helpful tips would be appreciated. I will compile responses and send back to the list. Thanks for any information, dacostaj@bu.edu