Dear All, A couple weeks ago, I asked the list for suggestions and opinions on methods for the purification of DNA from agarose gels. Thanks for all the great responses! Several people asked if I could summarize the results and send it to the list, so here it is. I decided to go with the Omega Ultra-Sep Gel Extraction Kit because it is very cost efficient (500 preps for ~USD $200). Tania King (who recommended this method, said "it's very cheap and the yield is very good - better than any other gel kit I've used." http://www.omegabiotek.com Other options include: 1. Roche High Pure PCR Purification kit - used for gel and direct PCR purification http://www.roche-applied-science.com 2. Bio Rad Freeze 'N Squeeze DNA Gel Extraction http://www.bio-rad.com 3. Millipore Montage Purification Kit - quick, only takes 10 mins and no need to melt the gel http://www.millipore.com 4. Healthcare illustra GFX PCR DNA and Gel Band Purification kit http://www.gelifesciences.com 5. Promega Wizard gel extraction kit http://www.promega.com 6. Zymoclean Gel DNA recovery Kit from Zymo Research http://www.zymoresearch.com 7. Qiagen Gel Extraction kits - multiple people reported having issues with the quality of the extracted products http://www.qiagen.com 8. "Pepsi's" (published by Travis Glenn, though I couldn't find the ref) - involves putting aquarium wool in 1mL pipette tips and adding the gel slice to the wool; spin inside a 1.5mL tube. Recovery varies, but very cheap (suggested by Joe Stanton) Stacy J. Mantooth Ecology and Evolutionary Biology Section School of Life Sciences University of Nevada, Las Vegas 4505 Maryland Parkway Las Vegas, NV 89154-4004 (702) 895-5373 / (702) 895-3956 (fax) mantooth@unlv.nevada.edu http://faculty.unlv.edu/mantooth/ mantooth@unlv.nevada.edu