Dear all Many thanks to all that replied to my request for a simulation software for genetic data. Many people asked to have a copy of the answers. Below there is my original question and all the replies. Thank you again to all. My question: I'm looking for a simulation software for genetic data that allows: - input genotpypic data - perform random mating simulation for several generations Easypop doesn't allow to input data file. I'm greateful to all for any help. REPLIES: Hi Daniele, You could try Nemo. A friend of mine built it and I have been using it extensively. There is functionality built into Nemo to read from an input file and to perform random mating simulations, but I am not sure how easy it is to construct an input file from scratch with your own data. I've copied Fred's email here, as he programmed it originally, so you might ask him. Good luck! Sam http://nemo2.sourceforge.net/ http://sourceforge.net/projects/nemo2/ Hi Daniele, it sounds like BOTTLESIM could be what you are looking for. It does however not model migration or mutation, only drift: http://chkuo.name/software/BottleSim.html Cheers, Frank Dear Daniele, The software HybridLab from Nielsen can do this and uses a genepop input file. Although initially devloped for hybridisation simulations, when using twice the same population, it creates random mating within this population. It is however not automated to run for several generations, requiring a script or manual uploading of the result file as input for every generation. If would be happy to get the other responses you get on this topic. buona fortuna ! ciao Greg references: http://www.dfu.min.dk/ffi/uk/populationgenetic/hybridlab/ http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1471-8286.2006.01433.x?cookieSet=1 Hi, Have you tried simuPOP (by Peng) or FPG. I have never used them, so Im not sure they can do what you ask. Peng and Kimmel 2005. simuPOP: a forward-time population genetics simulation environment BIOINFORMATICS APPLICATIONS NOTE 21(18), pages 3686-3687 Good luck Miguel Navascués FPG http://lifesci.rutgers.edu/~heylab/HeylabSoftware.htm#FPG Dear Daniele. Regarding your question at EvolDir: HYBRIDLAB might do the trick, except I'm not sure that it automatically can simulate over several generation. You may have to do a separate run for each generation and then use the output file from the previous simulated generation. Anyway, I've attached two references, so you can check them out Good luck Morten Allentoft Finally, I add to the list ManagedPop (Written by Kenneth Birnbaum, Charles M. Peters, Philip N. Benfey, and Rob DeSalle). It is a drift simulation that projects loss of allelic diversity in populations with overlapping generations that receive an input of progeny from a small (in terms of genetic identity) second population. Daniele.Porretta@uniroma1.it