Dear colleagues, Thank you so much for those who responded my question about convergence issue in MrBayes tree. Below is a summary of the responses. (1) AWTY software can be used ( http://king2.scs.fsu.edu/CEBProjects/awty/awty_start.php). (2) check the PSFR value (should approaching to 1). (3) check the standard deviation of split frequencies (should approaching to 0, less than 0.01) (4) Tracer software can be used (http://beast.bio.ed.ac.uk/Tracer) Haiwei Luo University of Georiga Haiwei Luo