Hi All, Here is the compiled list of all the responses that i have received. Hope it is useful to you. :o)) *Programs for sequence assembly:* * * Geneious 3.5 (www.geneious.com) PHRED/PHRAP applications available by request from the Green lab http://www.phrap.org/phredphrapconsed.html VectorNTI from Invitrogen features http://www.invitrogen.com/content.cfm?pageid=10071 Sequencher http://www.genecodes.com/ macvector (http://www.macvector.com/Products/macvectorassembler.html). Codon Code Aligner (www.codoncode.com) Vector NTI. it is free to academics. freeware BioEdit. http://www.mbio.ncsu.edu/BioEdit/bioedit.html SeqMan, EditSeq and SeqBuilder programs of the Lasergene/DNAStar software for assembly and translation and MacVector for alignments. (http://www.dnastar.com/products/lasergene.php) Se-Al http://tree.bio.ed.ac.uk/software/seal/ -- Regards Er. Rohini Bansal Ph.D. student Dr. Praveen Karanth's Lab CES, IISc, Bangalore-560012 India bansalrohini@gmail.com