here are the answers of kind Evoldir members who helped to identify valuable alternatives to Sequence Navigator Hi, I'm using the same G5 as you, and I solved such problem by installing the MacOS-9 Classic from the MacOS 10.3.8 installing package. Thus, when I start SeqNav up I get into a virtual OS9 and everything works Ok! Good luck, and have a nice day, Eric, eric.rivera-milla@uni-konstanz.de Hi there, we used to use sequence navigator when analysing data for MLST. As you have found, it has been rather left by the wayside. Nowadays we use the STARS program, which is a free staden interface developed for analysing polymorphisms within the same stretch of DNA (available from http://www.molbiol.ox.ac.uk/~paediat/stars/). It includes the ability to interrogate a database of alleles online and assign them to strains. I'm not sure if this would be useful for what you are doing (presumably with mycobacteria, yes?). You would need to work with it through an Xwindow on your new mac. Also install STADEN etc. Alternatively, the new version of MEGA (MEGA3 megasoftware.net) has the ability to analyse elctropherograms, align sequences etc. It's not ideal for these purposes but it might be better than worrying about 'surprising' sequence modifications. You would have to use a windows emulator on your mac, I'm afraid. Unfortunately, these are both either UNIX (STARS) or PC based (MEGA). But they are both free. I hope this helps. If anyone does write in with a way to extend the use of sequence navigator, please let me know! Best Bill Dr. W. P. Hanage, Imperial College Faculty of Medicine, Department of Infectious Disease Epidemiology, St Mary's Hospital, saw your post on EvolDir re. Sequence Navigator and OSX. I suggest to check out FinchTV by Geospiza (www.geospiza.com/finchtv) which is great for vieweing traces, and has the advantage to also view the raw data and do BLAST searches directly out of the program. 4Peaks (http://www.mekentosj.com/4peaks/) is also great for viewing and editing (more similar to Sequence Navigator I think). best of all: both are free! if you need a good OSX program for sequence editing and contig assembly, I suggest to check out Codon Code Aligner (www.codoncode.com), which is a great program (we are using it happily since more than a year) and has a very good licensing scheme, much more flexible than Sequencher, less than half the price (and no dongles), and very responsive suppport if you run into any trouble! Would be great if you could post all replies back on EvolDir. Good luck best wishes Gert Gert Wörheide Junior Professor for Geobiology Geoscience Centre Göttingen Dept. of Geobiology Goldschmidtstr.3 37077 Göttingen Germany Centre for Biodiversity and Ecology University of Göttingen phone: +49-(0)551 39 14 177 mobile: +49-(0)178 537 22 33 fax: +49-(0)551-39 79 18 -- Veronique Vincent Centre National de Reference des Mycobacteries Laboratoire de Reference des Mycobacteries Institut Pasteur 25 rue du Docteur Roux 75015 Paris Tel: 33 (0)1 45 68 83 60 FAX: 33 (0)1 40 61 31 18 vvincent@pasteur.fr vvincent@pasteur.fr