454 Titanium+ providers: Answers Dear Evoldir Community, thank you for your responses to a request about Titanium XL + providers I posted a few days ago. As several people asked me to share the results, find blow a compilation of helpful answers I received, and a list of companies which confirmed using XL+ chemistry. best, Gregor ----- Xl is only available for fragment libraries. For amplicons you are stuck with gs flx titanium for the foreseeable future P ----- Dear Gregor, The Centre for Genomic Research atUniversity ofLiverpool runs 2x 454xl+ machines. Both shotgun and amplicon libraries. Note that 1000bp is the absolute maximum read. Median is more like 700 - 750bp. Contact our centre manager, Dr Christiane Hertz-Fowler, if you'd like a quote or further details. chf@liv.ac.uk http://www.liv.ac.uk/genomic-research/ Best wishes, Steve ----- Dear Gregor, I've had your request for a 454 Titanium XL+ provider passed on to me from a colleague that is on the same email list as you. We do currently offer 454 Titanium XL+ sequencing and have for a few months now. However, these kits are currently not supported for amplicon sequencing (only for gDNA and cDNA libraries) and Roche strongly recommends that amplicons still be run using the Titanium XLR70 kits. I have run one short amplicon library as a test for a customer with the XL+ kits and the data was not nearly as good as has been obtained for his similar libraries sequenced with the XLR70 kits. Other service providers have said the same, the XL+ kits consistently give worse results for amplicons. This is due at least in part to the software not yet being updated/optimised for longer amplicons. I hope this you find this information helpful. Kind regards, Jason Koval -- *Ramaciotti Centre for Gene Function Analysis* Level 1, Biological Sciences Building (D26) The University of New South Wales NSW 2052 Telephone: +61 (2) 9385 1144 Facsimile: +61 (2) 9385 1277 http://www.ramaciotti.unsw.edu.au ----- Hi, we are in contact with LGC Genomics (through its Berlin facility) which offers at least 700 bp reads. www.lgcgenomics.com We have no practical experience with them yet === List of companies: FLX sequencing platform, Matís: www.matis.is ----- FGCZ Sequencing Team, Zurich: sequencing@fgcz.ethz.ch ----- Ramaciotti Centre, Sydney: http://www.ramaciotti.unsw.edu.au ----- University ofLiverpool,Liverpool: http://www.liv.ac.uk/genomic-research/ ----- LGC Genomics, Berlin: www.lgcgenomics.com ----- Research and Testing, Lubbock: http://researchandtesting.com/Sequencing.html Gregor Wachter