Thanks to everyone who sent me responses to my request for lists of selection experiments that were unsuccessful (i.e. no response). I am posting the final list here rather than responding to everyone individually, as there were a lot of responses. I believe the list below is accurate, but of course I'm responsible for any errors. And if I've missed anything, please email me. There is also one more, not listed, that I'm submitting for publication. Thanks, Jerry Experiments in which selection on at least one trait failed to achieve a response: Baer, C. F., and J. Travis. 2000. Direct and correlated responses to artificial selection on acute thermal stress tolerance in a livebearing fish. Evolution 54:238-244. Bradshaw, A. D. 1991. Genostasis and the limits to evolution. Phil. Trans. Roy. Soc. Lond. 333:289-305. Breuker CJ, Brakefield PM, 2003. Lack of response to selection for lower fluctuating asymmetry of mutant eyespots in the butterfly Bicyclus anynana. Heredity. 91(1):17-27. Collins, R. L. 1969. On inheritance of handedness 2. Selection for Sinistrality in Mice. Journal of Heredity 60:117-&. Collins, S., and G. Bell. 2004. Phenotypic consequences of 1,000 generations of selection at elevated CO2 in a green alga. Nature 431:566-569. Conner, J. K., and A. A. Agrawal. 2005. Mechanisms of constraints: the contributions of selection and genetic variance to the maintenance of cotyledon number in wild radish. Journal of Evolutionary Biology 18:238-242. Coyne, J. A. 1987. Lack of response to selection for directional asymmetry in Drosophila melanogaster. J. Heredity 78: 119. Hall, M., A. K. Lindholm, and R. Brooks. 2004. Direct selection on male attractiveness and female preference fails to produce a response. Bmc Evolutionary Biology 4:-. Hoffmann, A. A., R. J. Hallas, J. A. Dean, and M. Schiffer. 2003. Low potential for climatic stress adaptation in a rainforest Drosophila species. Science 301:100-102. Hulata G., G.W. Wohlfarth, and A. Halevy, 1986. Mass selection for growth rate in Nile tilapia (Oreochromis niloticus). Aquaculture, 57:1-4. Maughan, H., and W. L. Nicholson. 2004. Stochastic processes influence stationary-phase decisions in Bacillus subtilis. Journal of Bacteriology 186:2212-2214. Maynard Smith, J., and K. C. Sondhi. 1960. The genetics of a pattern. Genetics 45:1039-1050. Purnell, D. J., and J. N. Thompson. 1973. Selection for asymmetrical bias in a behavioral character of Drosophila melanogaster. Heredity 31:401-405. Tuinstra, E. J., G. Dejong, and W. Scharloo. 1990. Lack of response to family selection for directional asymmetry in Drosophila melanogaster : Left and right are not distinguished in development. Proceedings of the Royal Society of London Series B-Biological Sciences 241:146-152. Zijlstra, W.G., Kesbeke, F., Zwaan, B.J., and Brakefield, P.M. (2002). Protandry in the butterfly Bicyclus anynana. Evol. Ecol. Res., 4: 1229-40 Zijlstra, W. G., Steigenga, M.J., Brakefield, P.M., and Zwaan B.J. (2003). Simultaneous selection on two-components of life-history in the butterfly Bicyclus anynana. Evolution, 57: 1852-1862 Jerry Coyne