PhD project on gene expression and evolutionary dynamics in endangered small-mouthed salamanders We are seeking a PhD candidate to extend long-term efforts to understand the ecology, genomics, and conservation status of endangered small-mouthed salamanders and reliant unisexual salamanders on Pelee Island, Ontario, Canada. The salamander complex on Pelee Island is currently at risk because of combined impacts of habitat loss and kleptoparasitism by unisexual salamanders (see Bare et al. 2023. Oecologia 202: 807-818). Using our extensive dataset of thousands of genotyped salamanders, combined with ongoing field sampling and habitat suitability and connectivity analysis, the PhD candidate will explore one or more of the following: i) tools to increase genome-wide loci representation for higher resolution population genetics (RAD-seq); ii) amplification of corticosterone responsive genes as candidate genomic markers to evaluate transcriptomic stress; iii) genomotype/environment interactions across isolated populations; and jv) interactions between inbreeding and drift, elevated unisexual ploidy, and environmental stress. The work may culminate by modeling salamander population viability under selection pressures in contemporary landscapes that favour unisexual polyploids over small-mouthed salamanders. The student's research can be further tailored according to interests and expertise, e.g., investigating stress responses via transcriptomics, profiling for novel candidate stress genes, or relating genetic variance to phenotype. The funding package includes a competitive stipend, international tuition fee waiver, as well as coverage of all professional expenses. Successful candidates must have an MSc in Biology, Genetics, or a related field, and demonstrable evidence of peer-reviewed publication. Candidates should have experience in more than one of the following areas: population genetics, RADseq analysis, transcriptomics, evolutionary ecology, population modeling and amphibian fieldwork. Successful candidates will have a strong work ethic and interest in working collaboratively in a large and diverse research group. To apply, please send: i) cover letter highlighting relevant prior experience and interests corresponding to the above program priorities, ii) curriculum vitae, iii) unofficial academic transcript, and iv) contact information for 3 references. The successful candidate(s) will be co-supervised by Tom Hossie (www.thomashossie.ca) and Dennis Murray (www.dennismurray.ca) at Trent University and applications should be sent to thossie@trentu.caand dennismurray@trentu.ca. The graduate positions will begin in May 2024 or September 2024, and the postings will be closed as soon as suitable candidates are found. Dennis Murray CRC in Integrative Wildlife Conservation, Bioinformatics, and Ecological Modeling Director, Bioenvironmental Monitoring and Assessment graduate program Trent University Peterborough, ON CANADA www.dennismurray.ca dennis murray (to subscribe/unsubscribe the EvolDir send mail to golding@mcmaster.ca)