We are pleased to announce our upcoming Spring School in Bioinformatics (9th edition), which will take place online from 8-12 June. Course website: ( https://www.physalia-courses.org/courses-workshops/course68/ ) This five-day course will introduce participants to the fundamentals of Next Generation Sequencing (NGS) data analysis, combining conceptual lectures with extensive hands-on practical sessions. Participants will learn how to assess sequencing quality, assemble and annotate genomes, perform RNA-seq and differential gene expression analyses, and explore phylogenomics using NGS data. While the course will primarily focus on Illumina data, it will also cover the opportunities and requirements of long-read technologies such as Nanopore and PacBio. Throughout the week, participants will also gain practical experience with the Linux command line, as well as with Docker and software containers, which are widely used in bioinformatics to ensure reproducible analyses. The course is designed for researchers with a biological background who would like to gain practical experience in handling and analysing genomic and transcriptomic data. By the end of the training, participants will have a solid understanding of the strengths and limitations of NGS approaches and the tools commonly used in modern bioinformatics workflows. For the full list of our courses and workshops, please visit: ( https://www.physalia-courses.org/courses-workshops/course68/ ) Best regards, Carlo Carlo Pecoraro, Ph.D Physalia-courses DIRECTOR info@physalia-courses.org mobile: +49 17645230846 ( https://www.linkedin.com/in/physalia-courses-a64418127/ )