Dear all, 

We are pleased to announce the upcoming Physalia online course: Eukaryotic
(eDNA) Metabarcoding

Dates: 14-18 July 2025 Format: Online Course website: (
https://www.physalia-courses.org/courses-workshops/course4/ )

This hands-on course offers an in-depth overview of metabarcoding
techniques for the qualitative and quantitative assessment of biodiversity
in natural communities. Participants will explore practical pipelines
using short (Illumina)- and long (Nanopore)- data, and gain the skills
to design and analyze their own metabarcoding projects.

By the end of this course, participants will be able to:

Understand the principles and workflow of DNA metabarcoding, including
technical replication, sequencing depth, and methodological biases
Recognize good laboratory practices for sample collection, DNA extraction,
PCR, and library preparation Apply the APSCALE pipeline and BOLDigger
for raw read processing and taxonomic assignment Use TaxonTableTools
(TTT) for data filtering, alpha and beta diversity analysis, and result
visualization Explore eDNA metabarcoding using Nanopore sequencing and
understand its advantages and limitations in environmental applications
Build local reference databases and design metabarcoding primers for
custom applications

Confidently troubleshoot and design their own metabarcoding projects
with expert support This course is intended for researchers in ecology,
biodiversity, evolution and community biology, as well as those in
bioinformatics interested in ecological applications. Basic experience
with Unix commands and R is recommended.

Best regards, Carlo 

Carlo Pecoraro, Ph.D
Physalia-courses DIRECTOR
info@physalia-courses.org
mobile: +49 17645230846
( https://www.linkedin.com/in/physalia-courses-a64418127/ )

"info@physalia-courses.org" <info@physalia-courses.org>

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